Localisation of Donor and Acceptor Sites of NAD(P)H Dehydrogenase(S) Using “Inside-Out” and “Right-Side-Out” Plant Plasma Membrane Vesicles, and Characterization of Plasma Membrane-Bound b-Cytochromes
“Inside-out” and “right-side-out” plasma membranes (PM) were prepared from cauliflower as described for sugar beet leaf PM (1). Based on the latency of the K,Mg2+ ATPase, the “inside-out” fraction contained 51 % “inside-out” vesicles whereas the “right-side-out” fraction contained 10 % “inside-out” vesicles. Observe that with sugar beet leaf PM a much more extreme separation was obtained (1). The “inside-out” vesicles from cauliflower showed ATP-dependent proton uptake and were thus sealed.