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Localisation of Donor and Acceptor Sites of NAD(P)H Dehydrogenase(S) Using “Inside-Out” and “Right-Side-Out” Plant Plasma Membrane Vesicles, and Characterization of Plasma Membrane-Bound b-Cytochromes

  • Per Askerlund
  • Christer Larsson
  • Susanne Widell
Part of the NATO ASI Series book series (NSSA, volume 7)

Abstract

“Inside-out” and “right-side-out” plasma membranes (PM) were prepared from cauliflower as described for sugar beet leaf PM (1). Based on the latency of the K,Mg2+ ATPase, the “inside-out” fraction contained 51 % “inside-out” vesicles whereas the “right-side-out” fraction contained 10 % “inside-out” vesicles. Observe that with sugar beet leaf PM a much more extreme separation was obtained (1). The “inside-out” vesicles from cauliflower showed ATP-dependent proton uptake and were thus sealed.

Keywords

Electron Acceptor NADH Dehydrogenase Acceptor Site Cytoplasmic Side Plasma Membrane Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Larsson, C., Widell, S. & Sommarin, M. (1988) FEES Lett. 229: 289–292CrossRefGoogle Scholar
  2. 2.
    Kjellbom, P., Larsson, C., Askerlund, P., Schelin, C., & Widell, S. (1985) Photochem. Photobiol. 42: 779–783CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Per Askerlund
    • 1
  • Christer Larsson
    • 1
  • Susanne Widell
    • 1
  1. 1.Department of Plant PhysiologyLundSweden

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