Localisation of Donor and Acceptor Sites of NAD(P)H Dehydrogenase(S) Using “Inside-Out” and “Right-Side-Out” Plant Plasma Membrane Vesicles, and Characterization of Plasma Membrane-Bound b-Cytochromes

  • Per Askerlund
  • Christer Larsson
  • Susanne Widell
Part of the NATO ASI Series book series (NSSA, volume 7)

Abstract

“Inside-out” and “right-side-out” plasma membranes (PM) were prepared from cauliflower as described for sugar beet leaf PM (1). Based on the latency of the K,Mg2+ ATPase, the “inside-out” fraction contained 51 % “inside-out” vesicles whereas the “right-side-out” fraction contained 10 % “inside-out” vesicles. Observe that with sugar beet leaf PM a much more extreme separation was obtained (1). The “inside-out” vesicles from cauliflower showed ATP-dependent proton uptake and were thus sealed.

Keywords

Sugar NADPH NADH Spectrophotometry Oxidoreductase 

References

  1. 1.
    Larsson, C., Widell, S. & Sommarin, M. (1988) FEES Lett. 229: 289–292CrossRefGoogle Scholar
  2. 2.
    Kjellbom, P., Larsson, C., Askerlund, P., Schelin, C., & Widell, S. (1985) Photochem. Photobiol. 42: 779–783CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Per Askerlund
    • 1
  • Christer Larsson
    • 1
  • Susanne Widell
    • 1
  1. 1.Department of Plant PhysiologyLundSweden

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