Relationship between NAD+/NADH Levels and Animal Cell Growth
Studies have shown a close relationship between pyridine nucleotide levels and the proliferating stage of some tissues1. Also NAD(H) and NADP(H) pools have been studied as a function of growth in both normal and SV40 transformed 3T3 cells2,3. Decrease of NAD(H) levels in rat liver follows carcinogen (2-acetylaminofluorene) administration4. Therefore, it is reasonable to postulate that pyridine nucleotide pools are important in the control of cell division2,3,5. The regulation of the biosynthesis of pyridine nucleotides is complex since several alternative pathways have been described in mammalian cells3. Here, we show evidence that levels of pyridine nucleotides and relative redox states are altered in cells growing under the stimulation of growth factors, especially as a result of both short- and long-term treatments with external electron acceptors which react with the transplasma membrane redox system 5.
KeywordsHeLa Cell Pyridine Nucleotide NADH Concentration External Electron Acceptor Diferric Transferrin
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