Redox Agents which Modulate the Growth of Melanoma Cells: Evaluation of Mechanisms
The growth of many types of cell under the artificial conditions of in vitro culture is dependent on the attachment of the cells to a surface. The chemistry of the surface attachment can determine the response of the cells by modulating surface receptors for growth factors, by altering the strength of adhesion and thus overall cell surface area1 and in other ways which remain to be elucidated. An array of functional and structural changes in the surface of cells has been documented in cells undergoing the phenotypic expression of malignancy. The very basis of the commitment of cells to multiplication is the binding of growth factors to surface receptors as initial events in the cascade which leads to DNA synthesis and mitosis2. These initial events include phosphorylation of the growth factor receptors, and other proteins whose role in the cascade is yet to be determined, and very early changes in ion fluxes3, including egress of protons. It is clear that the plasma membrane is very actively involved in the events which determine whether a cell divides rapidly or slowly, differentiates, or languishes in a quiescent state. This study is directed towards an evaluation of some of the difficulties with attempts to define this association, and to evaluate some of the evidence that such electron flow may have a significant role in modulating cell attachment and replication.
KeywordsMelanoma Cell Melanoma Cell Line Human Melanoma Cell Buthionine Sulfoximine Lipid Soluble Antioxidant
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