Mechanisms of Ion Transport in Plants: K+ as an Example

  • William J. Lucas
  • Leon V. Kochian
Part of the NATO ASI Series book series (NSSA, volume 7)


Following Epstein’s pioneering research on the kinetics of K+ uptake into barley roots, in which he proposed that specific carriers are responsible for the movement of K+ across the plasma membrane, an extensive literature has developed in the general area of K+ transport in plant systems (for a review, see Kochfan and Lucas, 1988b). The development of hypothPGea on the actual mechanism(s) of K+ transport was strongly influenced by the advent of Mitchell’s chemiosmotic hypothesis. These models will be reviewed in an attempt to provide an appropriate background for an analysis of the involvement of transplasma membrane redox systems in influencing K+ transport.


Root Segment Redox System Barley Root Isolate Plasma Membrane Plasma Membrane ATPase 
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  1. Berridge, M.J., 1984, Inositol trisphosphate and diacylglycerol as second messengers. Biochem. J., 220: 345–360.PubMedGoogle Scholar
  2. Blatt, M.R., Rodriguez Navarro, A., and Slayman, C.L., 1987, Potassium-proton symport in Neurospora: Kinetic control by pH and membrane potential. J. Membr. Biol. 98: 169–189.PubMedCrossRefGoogle Scholar
  3. Boss, W.F., and Massel, M.O., 1985, PolyphosphoinositidPG are present in plant tissue culture cells. Biochem. Biophvs. Res. Commun., 132: 1018–1023.CrossRefGoogle Scholar
  4. Böttger, M., and Iüthen, H., 1986, Possible linkage between NADH-oxidation and proton secretion in Zea mays L. roots. J. Exp. Bot., 37: 666–675.CrossRefGoogle Scholar
  5. Briskin, D.P., 1986, Plasma membrane H+-transporting ATPase: role in potassium ion transports Physiol. Plantarum, 68: 159–163.CrossRefGoogle Scholar
  6. Briskin, D.P., and Leonard, R.T., 1982a, Partial characterization of a phosphorylated intermediate associated with the plasma membrane ATPase of corn root. Proc. Nat. Acad. Sci. USA, 79: 6922–6926.PubMedCrossRefGoogle Scholar
  7. Briskin, D.P., and Leonard, R.T., 1982b, Phosphorylation of the adenosine triphosphatase in a deoxycholate-treated plasma membrane fraction from corn roots. Plant Physiol., 70: 1459–1464.PubMedCrossRefGoogle Scholar
  8. Briskin, D.P., and Poole, R.J., 1983a, Plasma membrane ATPase of red beet forms a phosphorylated intermediate. Plant Physiol., 71: 507–512.PubMedCrossRefGoogle Scholar
  9. Briskin, D.P., and Poole, R.J., 1983b, Role of magnesium in the plasma membrane ATPase of red beet. Plant Physiol., 71: 969–971.PubMedCrossRefGoogle Scholar
  10. Buckhout, T.J., and Hrubec, T.C., 1986, Pyridine nucleotide-dependent ferricyanide reduction associated with isolated plasma membranes of maize (Zea mays L.) roots. Protoplasma, 135: 144–154.CrossRefGoogle Scholar
  11. Cantley, L.C., 1981, Structure and mechanism of the (Na,K) ATPase. Curr. Topics Bioenerg., 11: 201–237.Google Scholar
  12. Cheeseman, J.M., and Hanson, J.B., 1979a, Mathematical analysis of the dependence of cell potential on external potassium in corn roots. Plant Physiol., 63: 1–4.PubMedCrossRefGoogle Scholar
  13. Cheeseman, J.M., and Hanson, J.B., 1979b, Energy-linked potassium influx as related to cell potential in corn roots. Plant Physiol., 64: 842–845.PubMedCrossRefGoogle Scholar
  14. Cheeseman, J.M., and Hanson, J.B., 1980, Does active K+ influx to roots occur? Plant Sci. Lett., 18: 81–84.CrossRefGoogle Scholar
  15. Craig, T.A., and Crane, F.L., 1980, Evidence for a trans-plasma membrane electron transport system in plant cells. Proc. Indiana Acad. Sci., 90: 150–155.Google Scholar
  16. Craig, T.A., and Crane, F.L., 1981, Hormonal control of a transplasma membrane electron transport system in plant cells. Proc. Indiana Acad. Sci., 91: 150–154.Google Scholar
  17. De Quintero, M.R., and Hanson, J.B., 1984, Reactions of corn root tissue to calcium. Plant Physiol., 76: 403–408.PubMedCrossRefGoogle Scholar
  18. Epstein, E., Rains, D.W., and Elzam, O.E., 1963, Resolution of dual mechanisms of potassium absorption by barley roots. Proc. Nat. Acad. Sci. USA, 49: 684–692.PubMedCrossRefGoogle Scholar
  19. Fisher, J., and Hodges, T.K., 1969, Monovalent ion stimulated adenosine triphosphatase from oat roots. Plant Physiol., 44: 385–395.PubMedCrossRefGoogle Scholar
  20. Fisher, J.D., Hansen, D., and Hodges, T.K., 1970, Correlation between ion fluxes and ion-stimulated adenosine triphosphatase activity of plant roots. Plant Physiol., 46: 812–814.PubMedCrossRefGoogle Scholar
  21. Giannini, J.L., Gildensoph, L.H., Ruiz-Cristin, J., and Briskin, D.P., 1987, Isolation and characterization of sealed plasma membrane vesicles fran red beet (Beta vulgaris) storage tissue. Plant Physiol., 83: S–55.Google Scholar
  22. Glass, A.D.M., and Siddiqi, M.Y., 1982, Cation-stimulated H+ efflux by intact roots of barley. Plant Cell Environ., 5: 385–393.CrossRefGoogle Scholar
  23. Gronewald, J.W., and Hanson, J.B., 1982, Adenine nucleotide content of corn roots as affected by injury and subsequent washing. Plant Physiol., 69: 1252–1256.PubMedCrossRefGoogle Scholar
  24. Hodges, T.K., 1976, ATPases associated with membranes of plant cells, in: “Encyclopedia of Plant Physiology,” New Series, Vol. 2A, U. Iüttge and M.G. Pitman, eds., pp. 260–283, Springer-Verlag, Berlin.Google Scholar
  25. Ketchum, K.A., Cooper, E., Shrier, A., and Poole, R.J., 1987, Voltage-dependent whole cell currents observed in corn protoplasts. Plant Physiol., 83: S–112.Google Scholar
  26. Kochian, L.V., and Lucas, W.J., 1982, Potassium transport in corn roots. I. Resolution of kinetics into a saturable and linear component. Plant Physiol., 70: 1723–1731.PubMedCrossRefGoogle Scholar
  27. Kochian, L.V., and Lucas, W.J., 1985, Potassium transport in corn roots. III. Perturbation by exogenous NADH and ferricyanide. Plant Physiol., 77: 429–436.PubMedCrossRefGoogle Scholar
  28. Kochian, L. V., and Lucas, W.J., 1988a, Investigating root ion transport processes: an integrated experimental approach, in: “Redox Functions of the Eukaryotic Plasmalemma,” J. Ramirez, ed., C.S.I.C., Madrid, in press.Google Scholar
  29. Kochian, L. V., and Lucas, W.J., 1988b, Potassium transport in roots. Adv. Bot. Res., 15: in press.Google Scholar
  30. Kochian, L. V., Shaff, J.E., and Lucas, W.J., 1987, Electrogenic K uptake in corn roots: a lack of coLLelation with H+ efflux. Plant Physiol., 83: S–111Google Scholar
  31. Kochian, L.V., Xin-Zhi, J., and Lucas, W.J., 1985, Potassium transport in corn roots. IV. Characterization of the linear component. Plant Physiol., 79: 771–776.PubMedCrossRefGoogle Scholar
  32. Komar, E., Thann, M., and Maretzki, A., 1987, The oxidation of extracellular NADH by sugarcane cells: coupling to ferricyanide reduction, oxygen uptake and pH change. Planta, 170: 34–43.CrossRefGoogle Scholar
  33. Leonard, R.T., 1984, Membrane-associated ATPases and nutrient absorption by roots, in: “Advances in Plant Nutrition,” Vol. 1, P.B. Tinker and A. Läuchli, eds., pp. 209–240, Praeger Scientific, New York.Google Scholar
  34. Leonard, R.T., and Hanson, J.B., 1972, Induction and development of increased ion absorption in corn root tissue. Plant Physiol., 49: 430–435.PubMedCrossRefGoogle Scholar
  35. Leonard, R.T., and Hodges, T.K., 1973, Characterization of plasma membrane-associated adenosine triphosphatase activity of oat roots. Plant Physiol., 52: 6–12.PubMedCrossRefGoogle Scholar
  36. Lin, W., 1982a, Responses of corn root protoplasts to exogenous reduced nicotinamide adenine dinucleotide: oxygen consumption, ion uptake, and membrane potential. Proc. Nat. Acad. Sci. USA, 79: 3773–3776.PubMedCrossRefGoogle Scholar
  37. Lin, W., 1982b, Isolation of NADH oxidation system from the plasmalemma of corn root protoplasts. Plant Physiol., 70: 326–328.PubMedCrossRefGoogle Scholar
  38. Lin, W., 1984, Further characterization on the transport property of plasmalemma NADH oxidation system in isolated corn root protoplasts. Plant Physiol., 74: 219–222.PubMedCrossRefGoogle Scholar
  39. Lucas, W.J., and Kochian, L.V., 1988, Influence of exogenous NADH on K+ and H+ transport in corn roots, in: “Redox Functions of the Eukaryotic Plasmalemma,” J. Ramirez, ed., C.S.I.C., Madrid, in press.Google Scholar
  40. Marre, E., 1979, Fusicoccin: a tool in plant physiology. Annu. Rev. Plant Physiol., 30: 273–288.CrossRefGoogle Scholar
  41. Misra, P.C., Craig, T.A., and Crane, F.L., 1984, A link between transport and plasma membrane redox system(s) in carrot cells. J. Bioenerg. Biomembr. 16: 143–152.PubMedCrossRefGoogle Scholar
  42. Møller, I.M., and Lin, W., 1986, Membrane-bound NAD(P)H dehydrogenases in higher plant cells. Annu. Rev. Plant Physiol., 37: 309–334.CrossRefGoogle Scholar
  43. Morse, M.J., Crain, R.C., and Satter, R.L., 1987, Phosphatidylinositol cycle metabolites in Samanea saman pulvini. Plant Physiol., 83: 640–644.PubMedCrossRefGoogle Scholar
  44. Newman I.A., Kochian, L.V., Grusak, M.A., and Lucas, W.J., 1987, Fluxes of Ht and K+ in corn roots: characterization and stoichiometries using ion-selective microelectrodes. Plant Physiol., 84: 1177–1184.PubMedCrossRefGoogle Scholar
  45. Nishizuka, Y., 1984, The role of protein kinase C in cell surface signal transduction and tumor promotion. Nature, 308: 693–698.PubMedCrossRefGoogle Scholar
  46. Pitman, M.G., 1976, Ion uptake by plant roots, in: “Encyclopedia of Plant Physiology,” New Series, Vol. 2B, U. Lüttge and M.G. Pitman, eds., pp. 95–128, Springer-Verlag, New York.Google Scholar
  47. Poole, R.J., 1974, Ion transport and electrogenic props in storage tissue cells. Can. J. Bot., 52: 1023–1028.CrossRefGoogle Scholar
  48. Rincon, M., and Boss, W.F., 1987, myo-Inositol trisphosphate mobilizes calcium from fusogenic carrot (Daucus carota L.) protoplasts. Plant Physiol., 83: 395–398.Google Scholar
  49. Rodriguez Navarro, A., Blatt, M.R., and Slayman, C.L., 1986, A potassium- proton symport in Neurospora crassa. J. Gen. Physiol., 87: 649–674.PubMedCrossRefGoogle Scholar
  50. Rubinstein, B., and Stern, A.I., 1986, Relationship of transplasmalenmia redox activity to proton and solute transport by roots of Zea mays. Plant Physiol., 80: 805–811.PubMedCrossRefGoogle Scholar
  51. Sandelius, A., and Sommarin, M., 1986, Phosphorylation of phosphatidyl- inositols in isolated plant membranes. FEES Lett., 201: 282–286.CrossRefGoogle Scholar
  52. Schroeder, J.I., Hedrick, R. and Fernandez, J.M., 1984, Potassium-selec-tive single channels in guard cell protoplasts of Vicia faba. Nature, 312: 361–362.CrossRefGoogle Scholar
  53. Schroeder, J.I., Raschke, K., and Neher, E., 1988, Voltage-sensitive K+ channels in guard cell protoplasts. Prut. Nat. Acad. Sci. USA, in press.Google Scholar
  54. Serrano, R., 1984, Plasma membrane ATPase of fungi and plants as a novel type of proton pump. CUrr. Topics Cell Reg., 23: 87–126.Google Scholar
  55. Siddiqi, M.Y., and Glass, A.D.M., 1984, The influence of monovalent cations upon influx and efflux of calcium in barley (Hordeum vulgare). Plant Sci. Lett., 33: 103–114.CrossRefGoogle Scholar
  56. Thom, M., and Maretzki, A“, 1985, Evidence for a plasmalemma redox system in sugarcane. Plant Physiol., 77: 873–876.Google Scholar
  57. Vara, F., and Serrano, R., 1983, Phosphorylated intermediate of the ATPase of plant plasma membranes. J. Biol. Chem., 258: 5334–5336.PubMedGoogle Scholar
  58. Zocchi, G., and Hanson, J.B., 1982, Calcium influx into corn roots. Plant Physiol., 70: 318–319.PubMedCrossRefGoogle Scholar
  59. Zocchi, G., Rogers, S.A., and Hanson, J.B., 1983, Inhibition of proton pumping in corn roots is associated with increased phosphorylation of membrane proteins. Plant Sci. Lett., 31: 215–221.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • William J. Lucas
    • 1
  • Leon V. Kochian
    • 2
  1. 1.Department of BotanyUniversity of CaliforniaDavisUSA
  2. 2.United States Plant, Soil and Nutrition Laboratory, USDA-ARSCornell UniversityIthacaUSA

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