Modulation of Redox Reactions Involved in DNA Synthesis by Oxygen and Artificial Electron Acceptors
The study of the oxygen requirements for DNA synthesis in C3H 10T1/2 mouse embryonic fibroblasts offers several advantages for the understanding of mitogen-induced cell proliferation. Firstly, cell cycle kinetics have been well defined, and these cells are capable of being staged in Go/Gl so that the role of oxygen in different phases of the cell cycle can be evaluated. Further, these cells are capable of withstanding oxygen deprivation conditions necessary for removal of trace levels of dissolved oxygen which would mask the correct assessment of needs for oxygen.
KeywordsAmino Acid Incorporation Artificial Electron Acceptor Acid Insoluble Fraction External Electron Acceptor Uridine Monophosphate
Unable to display preview. Download preview PDF.
- 1.Mueller, G.C., Wertz, P.W., Kwong, C. H., Anderson, K. and Wrighton, S.A., Dissection of the early events in the activation of lymphocytes by 12–0-Tetradecanoylphorbol-13-acetate in “Carcinogenesis:Fundamental Mechanisms and Environmental Effects”. B. Pullman, P.O. Tso and H. Gelboin,eds. D.Reidel Publishing Co. Dordrecht, Netherlands(1980).Google Scholar
- 4.Crane, F. L., Sun, I. L., Clark, M.G., Grebing, C. and Low, H., 1985, Transplasma-membrane redox systems in growth and development. Biochim. Biophys. Acta 811: 233 (1985).Google Scholar
- 10.Cutry, A.F., Kinniburgh, A.J., Twardzik, D.R. and Wenner, C.E. Transforming growth factor alpha (TGFa) induction of cfos and c-myc expression in C3H 10T1/2 cells. Biochem. Biophys. Res. Comm. (in press, 1988).Google Scholar
- 11.Greenberg, M.E. and Ziff, E.B. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature 311: 433 (1984).Google Scholar
- 19.Pouyssegur, J., Chambard, J.J.C., Franchi, A., Paris, S. and van Obberghen-Schilling, E. Growth factor activation of an amiloride-sensitive Na+/H+ exchange system in quiescent fibroblasts: coupling to ribosomal protein S6 phosphorylation. Proc.Natl.Acad.Sci. 79: 3935 (1982).Google Scholar
- 21.Pouyssegur, J., Chambard, J.J.C., Franchi, A., L“Allemain, G., Paris, S. and van Obberghen-Schilling, E. Growth-factor activation of the Na+/H+ antiporter controls growth of fibroblasts by regulating intracellular pH. Cancer Cells (Cold Spring Harbor) 3: 409 (1985).Google Scholar