Modulation of Transferrin Receptor Gene Expression by Peptide Growth Factors and Role of the Receptor in Human Fibroblasts
The addition of serum or of the peptide growth factors PDGF, EGF and insulin in serum-free conditions to resting human HFL-1 fibroblasts stimulates cell proliferation and increases transferrin receptor mRNA levels and transferrin receptor numbers. The upregulation of transferrin receptor mRNA levels by growth factors is controlled at a level after transcription and is still observed when fibroblast proliferation is prevented by DNA synthesis inhibitors. Blocking of the transferrin receptor by 42/6 antibody does not influence the triggering of fibroblast proliferation by PDGF + EGF + insulin. Thus, in contrast to what has been observed with mitogen-stimulated human lymphocytes, transferrin receptor expression and upregulation in human diploid fibroblasts does not appear to be involved in the transduction of growth factor signals which trigger cell proliferation.
KeywordsIron Uptake Transferrin Receptor Fibroblast Proliferation Ferrous Citrate Peptide Growth Factor
Unable to display preview. Download preview PDF.
- 20.P. Basset, K. Yamauchi, and R. G. Crystal, Modulation of transferrin receptor mRNA levels in human fibroblasts by peptide growth factors, Fed. Proceed. 46: 1995 (1987).Google Scholar
- 21.I. S. Trowbridge, and R. A. Newnan, Monoclonal antibodies to transferrin receptors, in: “Receptors and Recognition, Series B, vol. 17, M.F. Greaves, Ed. Chapman and Hall, London (1984).Google Scholar