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Modulation of Transferrin Receptor Gene Expression by Peptide Growth Factors and Role of the Receptor in Human Fibroblasts

  • Paul Basset
Part of the NATO ASI Series book series (NSSA, volume 7)

Abstract

The addition of serum or of the peptide growth factors PDGF, EGF and insulin in serum-free conditions to resting human HFL-1 fibroblasts stimulates cell proliferation and increases transferrin receptor mRNA levels and transferrin receptor numbers. The upregulation of transferrin receptor mRNA levels by growth factors is controlled at a level after transcription and is still observed when fibroblast proliferation is prevented by DNA synthesis inhibitors. Blocking of the transferrin receptor by 42/6 antibody does not influence the triggering of fibroblast proliferation by PDGF + EGF + insulin. Thus, in contrast to what has been observed with mitogen-stimulated human lymphocytes, transferrin receptor expression and upregulation in human diploid fibroblasts does not appear to be involved in the transduction of growth factor signals which trigger cell proliferation.

Keywords

Iron Uptake Transferrin Receptor Fibroblast Proliferation Ferrous Citrate Peptide Growth Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Paul Basset
    • 1
  1. 1.Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l’INSERMFaculté de MédecineStrasbourg CédexFrance

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