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Transplasma Membrane Electron Transport Functions as a Ferric Reductase

  • H. Löw
  • A. Lindgren
  • F. L. Crane
  • I. L. Sun
  • W. Toole-Simms
  • D. J. Morré
Part of the NATO ASI Series book series (NSSA, volume 7)

Abstract

We have previously presented evidence that ferric ions and iron in diferric transferric can be reduced at the cell surface by the transplasma membrane electron transport system acting in conjuction with the transferrin receptor 1,2. In this paper we examine the reaction further especially in relation to the inhibition of both ferric citrate and diferric transferrin reduction by apotransferrin. We also consider the difference in activity found with different diferric transferrin preparations containing different amounts of loosely bound ferric iron. We will also consider the effects of argon, and cell transformation on the rate of ferric iron reduction in the presence and absence of diferric transferrin. The objective is to evaluate the extent to which loosely bound iron on transferrin can contribute to the formation of ferrous bathophenanthroline disulfonate (Fe(BPS)3) through the transmembrane electron transport system. We also show that iron reduction at acidic pH by the transmembrane enzyme is not inhibited by apotransferrin which would favor its role for ferric reduction in acidic endosomes.

Keywords

Ferric Citrate Ferric Ammonium Ammonium Citrate Ferric Ammonium Citrate Ll210 Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • H. Löw
    • 1
    • 2
    • 3
  • A. Lindgren
    • 1
    • 2
    • 3
  • F. L. Crane
    • 1
    • 2
    • 3
  • I. L. Sun
    • 1
    • 2
    • 3
  • W. Toole-Simms
    • 1
    • 2
    • 3
  • D. J. Morré
    • 1
    • 2
    • 3
  1. 1.Department of EndocrinologyKarolinska HospitalStockholmSweden
  2. 2.Department of BiologyPurdue UniversityW. LafayetteUSA
  3. 3.Department of Medical ChemistryPurdue UniversityW. LafayetteUSA

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