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Putative Amino Acid Transmitters in the Amygdala

  • O. P. Ottersen
  • B. O. Fischer
  • E. Rinvik
  • J. Storm-Mathisen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 203)

Abstract

The amygdala is involved in temporal lobe seizures, in man as well as in animal models (refs. in Ben-Ari, 1981), and is among the brain structures from which epileptiform seizures can be most effectively elicited by repeated electrical stimulation (Racine, 1981) or by topical injections of neuroexcitants (Ben-Ari et al., 1980; Tremblay et al., 1983). A better understanding of how the amygdala participates in these epileptic phenomena requires, among other things, more insight into its transmitter mechanisms. The amino acid transmitters gamma-aminobutyrate (GABA), glutamate (Glu), and aspartate (Asp) are of particular interest in this respect since it is assumed that they play decisive roles in the pathogenesis and sequelae of epilepsy (Meldrum, 1984). It is now time for increased efforts to unravel the amino acid transmitter mechanisms in the amygdala. First, the efferent and afferent fiber systems of the various amygdaloid subdivisions have recently been mapped in considerable detail (refs. in Ben-Ari, 1981), facilitating the undertaking and interpretation of experiments aimed at tracing transmitter-specific pathways. Second, new methods have been introduced to supplement those based on autoradiography of in vitro high affinity uptake of radiolabeled amino acids or on immunocytochemistry of glutamic acid decarboxylase (GAD), which have hitherto been the predominant histological techniques in amino acid transmitter research (for references see 9ttersen and Storm-Mathisen, 1984a). Thus, evidence has accumulated that D-[3H]Asp and [3H]GABA, injected in vivo, are selectively transported in axons of Glu/Asp-ergic and GABA-ergic neurons, respectively, leading to labeling of the parent cell bodies (Streit, 1980). Further, we have developed immunocytochemical techniques for the demonstration of GABA, Glu, and Asp, using antisera raised against the amino acids conjugated to protein by glutaraldehyde (Storm-Mathisen et al., 1983, 1986; Ottersen and Storm-Mathisen, 1984a,b, 1985; Storm-Mathisen and Ottersen, 1986). Antisera have also been raised against conjugates of taurine (Tau; Madsen et al., 1985; Ottersen et al., 1985), which is a more equivocal transmitter candidate than GABA, Glu, and Asp. In the present paper we report data obtained with amino acid immunocytochemistry and autoradiography of amino acid uptake and transport. Some of the results have been published elsewhere (Fischer et al., 1982; Ottersen and Storm-Mathisen, 1984a; Storm-Mathisen and Ottersen, 1986).

Keywords

Thalamic Nucleus Glutamic Acid Decarboxylase Stria Terminalis Amygdaloid Nucleus Amygdaloid Complex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baughman, R.W., and Gilbert, C.D., 1981, Aspartate and glutamate as possible neurotransmitters in the visual cortex, J. Neurosci., 1:427.Google Scholar
  2. Ben-Ari, Y., Kanazawa, I., and Zigmond, R.E., 1976, Regional distribution of glutamate decarboxylase and GABA within the amygdaloid complex and stria terminalis system of the rat, J. Neurochem., 26:1279.Google Scholar
  3. Ben-Ari, Y., Tremblay, E., and Ottersen, O.P., 1980, Injections of kainic acid into the amygdaloid complex of the rat: an electrographic, clinical and histological study in relation to the pathology of epilepsy, Neuroscience, 5: 515.Google Scholar
  4. Ben-Ari, Y., 1981, The Amvadaloid Complex,. Elsevier/North-Holland Biomedical Press, Amsterdam.Fischer, B.O., Ottersen, O.P., and Storm-Mathisen, J., 1982, Labelling of amygdalopetal and amygdalofugal projections after intra-amygdaloid injections of tritiated D-aspartate, Neuroscience, 7(Suppl.):S69.Google Scholar
  5. Fonnum, F., 1984, Glutamate: a neurotransmitter in mammalian brain, J. Neurochem„ 42:1.Google Scholar
  6. Heimer, L., 1978, The olfactory cortex and the ventral striatum, in: Limbic Mechanisms, K.E. Livingston and O. Hornykiewicz, eds., Plenum Press, New York, p. 95.Google Scholar
  7. Krettek, J.E., and Price, J.L., 1977, Projections from the amygdaloid complex to the cerebral cortex and thalamus in the rat and cat, J. Como. Neurol., 172:687.Google Scholar
  8. Krettek, J.E., and Price,J.L., 1978a, Amygdaloid projections to subcortical structures within the basal forebrain and brainstem in the rat and cat, J. Como. Neurol., 178:225.Google Scholar
  9. Krettek, J.E., and Price, J.L., 1978b, A description of the amygdaloid complex in the rat and cat with observations on intra-amygdaloid axonal connections, J. Como. Neurol., 178:255.Google Scholar
  10. Le Gal La Salle, G., 1976, Antidromic identification of amygdaloid multipolar neurons, Brain Res., 118: 479.Google Scholar
  11. Le Gal La Salle, G., Paxinos, G., Emson, P., and Ben-Ari, Y., 1978, Neuro- chemical mapping of GABAergic systems in the amygdaloid complex and bed nucleus of the stria terminalis, Brain Res., 155: 397.Google Scholar
  12. Madsen, S., Ottersen, O.P., and Storm-Mathisen, J., 1985, Immunocytochemical visualization of taurine: neuronal localization in the rat cerebellum, Neurosci. Lett., 60:255.Google Scholar
  13. McDonald, A.J., 1982, Cytoarchitecture of the central amygdaloid nucleus of the rat, J. Como. Neurol., 208:401.Google Scholar
  14. McDonald, A.J., 1984, Neuronal organization of the lateral and basolateral amygdaloid nuclei in the rat, J. Como. Neurol., 222:589.Google Scholar
  15. McDonald, A.J., 1985, Immunohistochemical identification of y-aminobutyric acid-containig neurons in the rat basolateral amygdala, Neurosci. Lett., 53:203.Google Scholar
  16. Meldrum, B., 1984, Amino acid neurotransmitters and new approaches to anticonvulsant drug action, Eoileosia, 25 (Supp1.2): 5140.Google Scholar
  17. Millhouse, 0.E, and DeOlmos, J., 1983, Neuronal configurations in lateral and basolateral amygdala, Neuroscience, 10: 1269.Google Scholar
  18. Ottersen, 0.P., and Ben-Ari, Y., 1979,. Afferent connections to the amygdaloid complex of the rat and cat. I. Projections from the thalamus, J. Como. Neurol., 187:401.Google Scholar
  19. Ottersen, 0.P., 1980, Afferent connections to the amygdaloid complex of the rat and cat: II. Afferents from the hypothalamus and the basal telencephalon, J. Como. Neurol., 194:267.Google Scholar
  20. Ottersen, 0.P., 1981, Afferent connections to the amygdaloid complex of the rat with some observations in the cat. III. Afferents from the lower brain stem, J. Como. Neurol., 202:335.Google Scholar
  21. Ottersen, 0.P., 1982, Connections of the amygdala of the rat. IV: Corticoamygdaloid and intraamygdaloid connections as studied with axonal transport of horseradish peroxidase, J. Como. Neurol., 205:30.Google Scholar
  22. Ottersen, 0.P., Fischer, B.O., and Storm-Mathisen, J., 1983, Retrograde transport of D-[H]aspartate in thalamocortical neurones, Neurosci. Lett.,42:19.Google Scholar
  23. Ottersen, O.P., and Storm-Mathisen, J., 1984a, Neurons containing or accumu- lating transmitter amino acids, ice: Handbook of Chemical Neuroanatomv,A. Björklund, T. Hökfelt, and M.J. Kuhar, eds., Elsevier/North-Holland, Amsterdam, p. 141.Google Scholar
  24. Ottersen, O.P., and Storm-Mathisen, J., 1984b, Glutamate- and GABA-containing neurons in the mouse and rat brain, as demonstrated with a new immunocytochemical technique, J. Como. Neurol,, 229:374.Google Scholar
  25. Ottersen, 0.P., Madsen, S., Meldrum, B.S., and Storm-Mathisen, J., 1985, Taurine in the hippocampal formation of the Senegalese baboon, Pardo, Paoip: an immunocytochemical study with an antiserum against conjugated taurine, Exo. Brain Res., 59:457.Google Scholar
  26. Ottersen, 0.P., and Storm-Mathisen, J., 1985, Different neuronal localization of aspartate-like and glutamate-like immunoreactivities in the hippocampus of rat, guinea pig, and Senegalese baboon (Paolo oaoio), with a note on the distribution of GABA, Neuroscience, 16: 589.Google Scholar
  27. Price, J.L., 1981, Toward a consistent terminology for the amygdaloid complex, in: The Amvadaloid Complex, Y. Ben-Ari, ed., Elsevier/NorthHolland Biomedical Press, Amsterdam. p. 13.Google Scholar
  28. Racine, R.J., 1981, Kindling: a model of amygdaloid epileptogenesis, in: The Amvgdaloid Complex. Y. Ben-Ari, ed., Elsevier/North-Holland Biomedical Press, Amsterdam, p. 431.Google Scholar
  29. Roberts, G.W., Woodhams, P.L., Polak, J.M., and Crow, T.J., 1982, Distribution of neuropeptides in the limbic system of the rat: the amygdaloid complex, Neuroscience, 7: 99.Google Scholar
  30. Smith, B.S., and Millhouse, O.E., 1985, The connections between the basolateral and central amygdaloid nuclei, Neurosci. Lett., 56:307.Google Scholar
  31. Stephan, H., 1975, Allocortex, Springer-Verlag, Berlin.CrossRefGoogle Scholar
  32. Sternberger, L.A., 1979, Immunocvtochemistrv, Second edition, John Wiley, New York.Google Scholar
  33. Storm-Mathisen, J., 1982, Amino acid compartments in hippocampus: an autoradiographic approach, in: Neurotransmitter Interaction and Comoartmentation, H.F. Bradford. ed., Plenum Press, New York, P. 395.Google Scholar
  34. Storm-Mathisen, J., Leknes, A.K., Bore, A.T., Vaaland, J.L., Edminson, P., Haug, F.-M.S., and Ottersen, 0.P., 1983, First visualization of glutamate and GABA in neurones by immunocytochemistry, Nature 301: 517.Google Scholar
  35. Storm-Mathisen, J., and Ottersen, 0.P., 1986, Antibodies against amino acid transmitters, in: Neurohistochemistry Today, P. Panula, H. Päivärinta, and S. Soinila, eds., Alan R. Liss, New York, in press.Google Scholar
  36. Storm-Mathisen, J., Ottersen, O.P., and Fu-long, T., 1986, Antibodies for the localization of excitatory amino acids, in: Excitatory Amino Acids, P.J. Roberts, J. Storm-Mathisen, and H.F. Bradford, eds., MacMillan, London, in press.Google Scholar
  37. Streit, P., 1980, Selective retrograde labeling indicating the transmitter of neuronal pathways, J. Comp. Neurol., 191:429.Google Scholar
  38. Taxt, T., and Storm-Mathisen, J., 1984, Uptake of D-aspartate and L-glutamate in excitatory axon terminals in hippocampus: autoradiographic and biochemical comparison with y-aminobutyrate and other amino acids in normal rats and in rats with lesions, Neuroscience, 11: 79.Google Scholar
  39. Tremblay, E., Ottersen, 0.P., Rovira, C., and Ben-Ari, Y., 1983, Intraamiygdaloid injections of kainic acid: regional metabolic changes and their relation to the pathological alterations, Neuroscience, 8: 299.Google Scholar
  40. Walker, J.E., and Fonnum, F., 1983, Regional cortical glutamergic and aspartergic projections to the amygdala and thalamus of the rat, Brain Res., 267: 371.Google Scholar
  41. Wiklund, L., Toggenburger, G., and Cuénod, M., 1982, Aspartate: possible neurotransmitter in cerebellar climbing fibers, Science, 216: 78.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • O. P. Ottersen
    • 1
  • B. O. Fischer
    • 1
  • E. Rinvik
    • 1
  • J. Storm-Mathisen
    • 1
  1. 1.Anatomical InstituteUniversity of OsloOslo 1Norway

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