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A Patch-Clamp Study of Excitatory Amino Acid Activated Channels

  • P. Ascher
  • L. Nowak
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 203)

Abstract

Although the introduction of the patch-clamp techniques (Neher and Sakmann, 1976; Hamill et al., 1981) has been universally recognized as a major breakthrough in the biophysical study of ion channels, these techniques have not yet become a standard tool for pharmacological studies. We shall briefly describe below some of the results that we have obtained, using ‘outside-out’ patches of mouse central neurons (Nowak et al., 1984), in the characterization of excitatory amino acid (EAA) receptors and channels. In this description, we shall not develop the biophysical results (and in particular the description of kinetics of the channels) but shall concentrate on the results of direct pharmacological and physiological relevance.

Keywords

Excitatory Amino Acid Glutamate Concentration Mammalian Central Nervous System Single Channel Current NMDA Channel 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • P. Ascher
    • 1
  • L. Nowak
    • 2
  1. 1.Laboratoire de NeurobiologieEcole Normale SupérieureParis Cedex 05France
  2. 2.Department of PharmacologyCornell University Veterinary CollegeIthacaUSA

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