A Patch-Clamp Study of Excitatory Amino Acid Activated Channels
Although the introduction of the patch-clamp techniques (Neher and Sakmann, 1976; Hamill et al., 1981) has been universally recognized as a major breakthrough in the biophysical study of ion channels, these techniques have not yet become a standard tool for pharmacological studies. We shall briefly describe below some of the results that we have obtained, using ‘outside-out’ patches of mouse central neurons (Nowak et al., 1984), in the characterization of excitatory amino acid (EAA) receptors and channels. In this description, we shall not develop the biophysical results (and in particular the description of kinetics of the channels) but shall concentrate on the results of direct pharmacological and physiological relevance.
KeywordsExcitatory Amino Acid Glutamate Concentration Mammalian Central Nervous System Single Channel Current NMDA Channel
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