Blockade by D-Aminophosphonovalerate or Mg2+ of Excitatory Amino Acid-Induced Responses on Spinal Motoneurons in Vitro

  • A. Nistri
  • A. E. King
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 203)


L-glutamate and its structurally-related analogs are known to evoke strong excitation of vertebrate central neurons (Nistri and Constanti, 1979; Puil, 1981). Pharmacological studies have shown that there are probably distinct receptor populations mediating responses to different excitatory amino acids (Watkins and Evans, 1981; McLennan, 1983): one receptor class is preferentially activated by N-methyl-D-aspartate (NMDA) while a separate receptor type selectively binds quisqualate. While it is not fully clear if kainate operates via a third receptor system, it seems that the endogenously occurring amino acids L-glutamate and L-aspartate have mixed agonist properties being able to bind NMDA as well as quisqualate receptors.


Excitatory Amino Acid Spinal Motoneuron Excitatory Amino Acid Receptor EPSP Amplitude Control Ringer 
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  1. Arenson, M.S., Berti, C., King, A.E., and Nistri, A., 1984, The effect of D-a-aminoadipate on excitatory amino acid responses recorded intracellularly from motoneurones of the frog spinal cord, Neurosci. Lett., 49: 99.Google Scholar
  2. Arenson, M.S., and Nistri, A., 1985, The effects of potassium channel blocking agents on the responses of in vitro frog motoneurones to glutamate and other excitatory amino acids: an intracellular study, Neuroscience, 14: 317.PubMedCrossRefGoogle Scholar
  3. Ault, B., Evans, R.H., Francis, A.A., Oakes, D.J., and Watkins, J.C., 1980, Selective depression of excitatory amino acid-induced depolarizations by magnesium ions in isolated spinal cord preparations, J. Physiol., 307: 413.PubMedGoogle Scholar
  4. Barrett, E.F., and Barret, J.N., 1976, Separation of two voltage sensitive potassium currents and the demonstration of a tetrodotoxin-resistant calcium current in frog motoneurones, J. Physiol., 255: 737.PubMedGoogle Scholar
  5. Bührle, C.P., and Sonnhof, U., 1983, The ionic mechanisms of excitatory action of glutamate upon the membranes of motoneurones of the frog, Pflügers Arch., 396: 154.PubMedCrossRefGoogle Scholar
  6. Chesselet, M.F., 1984, Presynaptic regulation of neurotransmitter release in the brain, Neuroscience, 12: 347.PubMedCrossRefGoogle Scholar
  7. Corradetti, R., King, A.E., Nistri, A., Rovira, C., and Sivilotti, L., 1985, Pharmacological characterization of D-aminophosphonovaleric acid antagonism of amino acid and synaptically evoked excitations on frog motoneurones in vitro: an intracellular study, Br. J. Pharmacol., 86: 19.Google Scholar
  8. Crunelli, V., and Mayer, M.L., 1984, Mg2+ dependence of membrane resistance increases evoked by NMDA in hippocampal neurones, Brain Res., 311: 392.PubMedCrossRefGoogle Scholar
  9. Davidoff, R.A., and Adair, R., 1975, High affinity amino acid transport by frog spinal cord slices, J. Neurochem., 24: 545.PubMedCrossRefGoogle Scholar
  10. Erulkar, S.D., Dambach, G.E., and Mender, D., 1974, The effect of magnesium at motoneurones of the isolated spinal cord of the frog, Brain Res., 66: 413.CrossRefGoogle Scholar
  11. Evans, R.H., Francis, D.A., Jones, A.W., Smith, D.A.S., and Watkins, J,C., 1982, The effects of a series of w-phosphonic a-carboxylic amino acids on electrically evoked and excitant amino acid-induced responses in isolated spinal cord preparations, Br. J. Pharmacol., 75: 65.Google Scholar
  12. Ferkany, J.W., and Coyle, J.T., 1983, Kainic acid selectively stimulates the release of endogenous excitatory amino acids, J. Pharmacol. Exp. Ther., 225: 399.Google Scholar
  13. Johnston, G.A.R., and Lodge, D., 1984, Ketamine and magnesium selectively block the N-methylaspartate-evoked release of acetylcholine from rat cortex slices in vitro, J. Physiol., 349: 15 P.Google Scholar
  14. Mayer, M.L., and Westbrook, G.L., 1984, Mixed-agonist action of excitatory amino acids on mouse spinal cord neurones under voltage clamp, J. Physiol. 354: 29.PubMedGoogle Scholar
  15. Mayer, M.L., and Westbrook, G.L., 1985, The action of N-methyl-D-aspartic acid on mouse spinal neurones in culture, J. Physiol., 361: 65.PubMedGoogle Scholar
  16. McLennan, H., 1983, Receptors for the excitatory amino acids in the mammalian central nervous system, Progr. Neurobiol., 20: 251.Google Scholar
  17. Nistri, A., and Constanti, A., 1979, Pharmacological characterization of different types of GABA and glutamate receptors in vertebrates and invertebrates, Progr. Neurobiol., 13: 117.Google Scholar
  18. Nistri, A., and Arenson, M.S., 1983, Differential sensitivity of spinal neurones to amino acids: an intracellular study on the frog spinal cord, Neuroscience, 8: 115.PubMedCrossRefGoogle Scholar
  19. Nistri, A., Arenson, M.S., and King, A., 1985, Excitatory amino acid-induced responses of frog motoneurones bathed in low Na+ media: an intracellular study, Neuroscience, 14: 921.PubMedCrossRefGoogle Scholar
  20. Nowak, L., Bregestovski, P., Ascher, P., Herbet, A., and Prochiantz, A., 1984, Magnesium gates glutamate-activated channels in mouse central neurones, Nature, 307: 462.PubMedCrossRefGoogle Scholar
  21. Puil, E., 1981, S-Glutamate: its interactions with spinal neurons, Brain Res. Rev., 3: 229.Google Scholar
  22. Roberts, P.J., and Anderson, S.D., 1979, Stimulatory effect of L-glutamate and related amino acids on [3H] dopamine release from rat striatum: an in vitro model for glutamate actions, J. Neuroohem., 32: 1539.CrossRefGoogle Scholar
  23. Watkins, J.C., and Evans, R.H., 1981, Excitatory amino acid transmitters, Ann. Rev. Pharmacol. Toxicol., 21: 165.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • A. Nistri
    • 1
  • A. E. King
    • 1
  1. 1.Department of Pharmacology, St. Bartholomew’s HospitalMedical College, University of LondonLondonGreat Britain

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