Monoclonal Antibodies in the Analysis of Drosophila Development
We are attempting to approach the molecular mechanisms involved in Drosophila development by using monoclonal antibodies raised against the surfaces of imaginal discs. It is generally acknowledged that the cell surface plays a major role in development. Surface components have been implicated, for example, in tissue induction (Saxen, 1977) and in cell-cell recognition (Moscona, 1975; Marchase et al., 1975). However, with one or two exceptions (Burger, 1977), little is known of the molecular interactions involved in these processes. It is not clear whether all, or only some, classes of surface molecules — proteins, glycoproteins, glycolipids and so on — participate. Nor do we know whether cellular discrimination stems from qualitative or quantitative changes in these components, or even whether the molecules are likely to be major or minor constituents of the surface. Possessing a set of specific “tags” for surface molecules should enable us to approach these questions. One advantage of the monoclonal antibody technique over conventional immunological approaches is that in principle even minor surface components can be specifically labelled.
KeywordsBasal Lamina Imaginal Disc Disc Cell Wing Disc Drosophila Development
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