Analysis of the Cornea of the Eye with the Electron Microprobe
Progress in techniques of biological tissue preparation for microprobe analysis has allowed the use of the probe in the study of experimental band keratopathy, in which calcium has precipitated in the tissues of the cornea. The condition was induced in rabbits by one of two methods: overdosing with vitamin D in rabbits with uveitis, and intravitreal injection of polyethylene sulfonic acid. In each case, a band of calcium appeared beneath the outer corneal epithelium, approximately 50 µ in thickness, in ca. 100 times normal concentration. The calcium concentration in the remainder of the cornea was normal, with the exception of minute areas of high concentration. Phosphorus was present in the band in high concentrations, but was not found in the smaller calcium-rich areas.
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