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Yeast as a Model for Studying DNA-Alterations and Biological Response Induced by Alkylating Anti-Cancer Drugs: Effects of Cyclophosphamide

  • Reinhard Fleer
  • Martin Brendel
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 40)

Summary

Activated cyclophosphamide (CP) induces DNA interstrand cross-links and DNA strand-breakage in the chromosomes of yeast. In contrast to the post-incubation reaction of other polyfunctional alkylating agents, a further increase of cross-links after treatment with activated CP was hardly detectable. This is possibly due to the observed continued DNA fragmentation which decreases the sensitivity of the cross-link assay. Cell killing by non-activated CP depends on the function of DNA repair as well as on the mode of cellular energy metabolism. At concentrations 1000-fold higher than those used for activated CP it induces DNA single- as well as double-strand breaks. In contrast, no induction of interstrand cross-links could be detected.

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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Reinhard Fleer
    • 1
  • Martin Brendel
    • 1
  1. 1.Institut für MikrobiologieJ.W.Goethe-UniversitätFrankfurt/M.Germany

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