Effects of Platinum Coordination Complexes on Mutants of Escherichia coli Defective in DNA Repair and Genetic Recombination
The effect of three platinum coordination complexes on wild type and repair deficient mutant of Escherichia coli has been investigated. The antitumor drug cis-platinum (II) diamminodichloride (cis-PDD) produces an inhibition of DNA synthesis and reduces the viability of excision repair and recombination defective mutants. The trans isomer (trans-PDD) has less effect than cis-PDD on all of these mutants while [Pt(dien)C1]C1 has no effect. Incorporation of radioactive precursors under non-replicating conditions and density gradient centrifugation analysis of DNA indicate that repair synthesis occurred after treatment of wild type cells with cis-PDD or trans-PDD.
Unable to display preview. Download preview PDF.
- 2.D.J. Beck and R.R. Brubaker, Mutagenic properties of cis-Platinum diaminodichloride in Escherichia coli, Mut. Res. 27: 181 (1975).Google Scholar
- 5.J.J. Roberts and A.J. Thompson, The mechanism of action of antitumor platinum compounds, in Prog. Nucl. Acid Res. and Mol. Biol. 21:71 (1979).Google Scholar
- 10.P.C. Hanawalt and P.K. Cooper, Determination of repair replication in vivo in Methods in Enzymology, 21: 221 (1971).Google Scholar