Heteroduplex DNA in Bacteriophage T1 after Ionizing Radiation
Most of the DNA lesions induced by ionizing radiation are caused indirectly by the action of water radiolysis products. In biological active systems however, hydroxyl- and peroxy radicals are more likely to react with cellular radical scavangers and the formation of DNA lesions may to a greater extent be mediated by the direct action of radiation. The biological and biochemical effects after exposure of a cell to a damaging agent result from the spectrum of lesions and their distribution in the genome. It seems evident, that bases per se are more involved in lesion formation than phosphates and sugars and in addition it is extremely difficult to select individual lesions among the complexity of modified purines and pyrimidines. One common expression of base damages result — depending on their intergenomic location — in helix distortion, interfered with restricted base stacking.
KeywordsIonize Radiation Nuclease Digestion Bulky Lesion Phage Head Water Radiolysis Product
Unable to display preview. Download preview PDF.
- 5.P. Cerutti, “DNA Repair Mechanisms”, P.C. Hanawalt, E.C. Friedberg, C.F. Fox,eds., Academic Press, New York (1978).Google Scholar
- 6.J.J. Weiss, “ Progress in Nucleic Acid Research and Molecular Biology”, J.N. Davidson and W.E.Cohn, eds., Academic Press, New York (1964).Google Scholar
- 7.M.C. Paterson, “DNA Repair Mechanisms”, P.C. Hanawalt, E.C.Friedberg, C.F. Fox, eds., Academic Press, New York (1978).Google Scholar