Advertisement

Role of Mammalian DNA Polymerases in Replication and Repair

  • Hans Krokan
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 40)

Abstract

Replication of eukaryotic nuclear DNA is discontinuous at two levels; it takes place within numerous replicons, and within each. replicon at least one of the two nascent DNA strands is made discontinuously by formation of small (average 135 nucleotides) primary pieces which are subsequently ligated (reviewed in ref. 1–6). Although there is no definite proof for the participa tion in DNA replication of any known enzyme activity, this model suggests that a minimum of six proteins are involved. These are an unwinding protein (DNA dependent ATPase?), an RNA polymerase (“primase”? RNA pol I?), a DNA polymerase (DNA pol α?), an RNAse (RNAse H?), a gap-filling DNA polymerase (DNA pol α?) and a DNA ligase (DNA ligase I? DNA ligase II?), (Fig. 1). The enzymology of mammalian DNA repair is very complex, and even less well understood than that of DNA replication.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Kornberg, A. (1980) In “DNA Replication”, W.H. Freeman.Google Scholar
  2. 2.
    DePamphilis, M.L. & Wassarman, B.M. (1980) Ann. Rev. Biochem. 49: 627CrossRefGoogle Scholar
  3. 3.
    Weissbach, A. (1979) Arch. Biochem. Biophys, 198: 386.CrossRefGoogle Scholar
  4. 4.
    Cremisi, C. (1979) Microbiol. Rev. 43: 297.PubMedPubMedCentralGoogle Scholar
  5. 5.
    Seale, R.L. (1978) In “The Cell Nucleus”, 4, (Bush, H., Ed.) pp. 155–172 Academic Press, Inc. N.Y., London and San Francisco.Google Scholar
  6. 6.
    Sheinin, R. & Humbert, J. (1978) Ann. Rev. Biochem. 47: 277.CrossRefGoogle Scholar
  7. 7.
    Bollum, F. (1975) In “Progr. Nucl. Acid. Res. and Mol. Biol.”, 15:109. Ed. W.E. Cohn, Academic Press, N.Y., London and San Francisco.Google Scholar
  8. 8.
    Hübscher, U., Kuenzle, C.C. & Spadari, S. (1979) Proc. Natl. Acad. Sci. 76: 2316.CrossRefGoogle Scholar
  9. 9.
    Chen, Y.C., Bohn, E.W., Planck, S.R. & Wilson, S.H. (1979) J. Biol. Chem. 254: 11678.PubMedGoogle Scholar
  10. 10.
    Byrnes, J.J. & Black, V.L. (1978) Biochemistry, 17: 4226.CrossRefGoogle Scholar
  11. 11.
    Hesslewood, I.P., Holmes, A.M., Wakeling, W.F. & Johnston, I.R. (1978) Eur. J. Biochem. 84: 123.CrossRefGoogle Scholar
  12. 12.
    Fisher, P.A. & Korn, D. (1979). J. Biol. Chem. 254: 11033.PubMedGoogle Scholar
  13. 13.
    Grummt, F., Waltl, G., Jantzen, H.M., Hamprecht, K., Huebscher, V. & Kuenzle, C. (1979) Proc. Natl. Acad. Sci. USA, 76: 6081.CrossRefGoogle Scholar
  14. 14.
    Wilson, S.H., Matuskage, A., Bohn, E.W., Chen, Y.C. & Sivarajan, M. (1977) Nucl. Acids Res. 4: 3981.CrossRefGoogle Scholar
  15. 15.
    Rapaport, E. & Samecnik, P. (1976) Proc. Natl. Acad. Sci. USA, 73: 3984.CrossRefGoogle Scholar
  16. 16.
    Grummt, F. (1978) Proc. Natl. Acad. Sci. USA, 75: 371.CrossRefGoogle Scholar
  17. 17.
    Weissbach, A., (1977) Ann. Rev. Biochem. 46: 25.CrossRefGoogle Scholar
  18. 18.
    Foster, D.N. & Gurney, T. (1976) J. Biol. Chem. 251: 7893.PubMedGoogle Scholar
  19. 19.
    Lynch, W.E., Surrey, S. & Lieberman, I. (1975) J. Biol. Chem. 250: 8179.PubMedGoogle Scholar
  20. 20.
    Herrick, G., Spear, B. and Veomett, G. (1976) Proc. Natl. Acad. Sci. USA, 73: 1136.CrossRefGoogle Scholar
  21. 21.
    Korn, D., Eichler, D.C., Fisher, P.A. & Wang, T.S.F. (1977) In “DNA Synthesis, Present and Future”, pp. 517–558, ed. Molineux, I. & Kohiyama, M. Plenum Press, N.X. & London.CrossRefGoogle Scholar
  22. 22.
    Su, R.T. & DePamphilis, M.L. (1976) Proc. Natl. Acad. Sci., USA 73: 3466.CrossRefGoogle Scholar
  23. 23.
    Edenberg, H.J., Anderson, S. & DePamphilis, M.L. J. Biol. Chem. 253: 3273.Google Scholar
  24. 24.
    Ikegami, S., Taguchi, T., Ohashi, M., Oguro, M., Nagano, H. & Mano, Y. (1978) Nature, 275: 458.CrossRefGoogle Scholar
  25. 25.
    Wist, E. &:Prydz, H. (1979) Nucl. Acids. Res. 4: 1583.CrossRefGoogle Scholar
  26. 26.
    Krokan, H., Schaffer, P. & DePamphilis, M.L. (1979) Biochemistry, 18: 4431.CrossRefGoogle Scholar
  27. 27.
    Wist, E. (1979) Biochim. Biophys. Acta 562: 62.CrossRefGoogle Scholar
  28. 28.
    Oguro, M., Suzuki-Hori, C., Nagano, H., Mano, Y. & Ikegami, S. (1979) Eur. J. Biochem. 97: 603.CrossRefGoogle Scholar
  29. 29.
    Wright, G.E., Baril, E.F. & Brown, N.C. (1980) Nucl. Acids. Res. 8: 99.CrossRefGoogle Scholar
  30. 30.
    DePamphilis, M.L., Anderson, S., Bar-Shavit, R., Collins, E., Edenberg, H., Herman, T., Karas, B., Kaufmann, G. Krokan, H., Shelton, E., Su, R., Tapper, D. and Wassarman, P.M. (1978) Cold Spring. Harb. Symp. Quant. Biol. 43: 679.CrossRefGoogle Scholar
  31. 31.
    Weaver, D., Krokan, H. & DePamphilis, M.L. (1980) J. Supramol. Str., Suppl. 4, Abs. 895: 333.Google Scholar
  32. 32.
    DePamphilis, M.L., Anderson, S., Cusick, M., Hay, R., Herman, T., Krokan, H., Shelton, E., Tack, L., Tapper, D., Weaver, D. & Wassarman, P.M. (1980) In Mechanistic Studies of DNA Replication and Genetic Recombination ICN UCLA Symposia on Molecular and Cellular Biology“ 19, in press.CrossRefGoogle Scholar
  33. 33.
    Hübscher, U., Kuenz.le, C.C. & Spadari, S. (1977) Eur. J. Biochem. 81: 249.CrossRefGoogle Scholar
  34. 34.
    Bertazzoni, U., Scovassi, A.I. & Brun, G. (1977) Eur. J. Biochem. 81: 237.CrossRefGoogle Scholar
  35. 35.
    Krauss, S.W. & Linn, S. (1980) Biochemistry 19: 220.CrossRefGoogle Scholar
  36. 36.
    Knopf, K.-W., Yamada, M. & Weissbach, A. (1980) Biochemistry, 15: 4540.CrossRefGoogle Scholar
  37. 37.
    Robert-Guroff, M., Schrecker, A.W., Brinkmann, B.J. & Gallo, R.C. (1977). Biochemistry, 16: 2866.Google Scholar
  38. 38.
    van der Vliet, P.C. & Kwant, M.M. (1978) Nature, 276; 532.CrossRefGoogle Scholar
  39. 39.
    Habara, A., Kano, K., Nagano, H., Mano, Y., Ikegami, S. & Yamashita, T. (1980) Biochem. Biophys. Res. Comm. 92: 8.CrossRefGoogle Scholar
  40. 40.
    Green, M., Arens, M.Q., Yamashita, T., Wold, W.S.M. & Brackmann, K.H. (1978) Cold Spring Harb. Symp. Quant. Biol. 43: 755.CrossRefGoogle Scholar
  41. 41.
    Longiaru, M., Ikeda, J.-E., Jarkovsky, Z., Horwitz, M. (1979) Nucl. Acids Res. 6: 3369.Google Scholar
  42. 42.
    Bjursell, G. (1978) J. Virol. 26: 136.PubMedPubMedCentralGoogle Scholar
  43. 43.
    Denhardt, D.T., Kowalski, J. & Miyamoto, C. (1978) Cold Spring Harb. Symp. Quant. Biol. 43: 243.CrossRefGoogle Scholar
  44. 44.
    Bjursell, G., Gussander, E. & Lindahl, T. (1979) Nature, 280: 420.CrossRefGoogle Scholar
  45. 45.
    Butt, T.R., Wood, W.M., McKay, E.L. & Adams, R.L.P. (1978) Biochem. J. 173: 309.CrossRefGoogle Scholar
  46. 46.
    Hobart, P.M. & Infante, A.A. (1978) J. Biol. Chem. 253: 8229.PubMedGoogle Scholar
  47. 47.
    In “DNA Repair Mechanisms”,(1978) (Hanawalt, P.C., Friedberg, E.C. & Fox, C.F. Eds.1. Academic Press, N.Y., San Francisco, London.Google Scholar
  48. 48.
    Bertazzoni, U., Stefanini, M., Pedrali-Noy, G., G.ullotto, E., Nuzzo, F., Falaschi, A. & Spadari, S. (1976) Proc. Natl. Acad. Sci. USA, 73: 7888.CrossRefGoogle Scholar
  49. 49.
    Ciarrocchi, G., Jose, J.G. & Linn, S. (1979) Nucl. Acids. Res. 7: 1205.CrossRefGoogle Scholar
  50. 50.
    Hanaoka, F., Kato, H., Ikegami, S., Ohashi, M. & Yamada, M. (1979) Biochem. Biophys. Res. Comm. 87: 575CrossRefGoogle Scholar
  51. 51.
    Berger, N., Kusohara, K.K., Petzold, S.J. & Sikurski, G.W. (1979) Biochem. Biophys. Res. Comm. 89: 218.CrossRefGoogle Scholar
  52. 52.
    Wawra, E. & Dolejs, I. (1979) Nucl. Acids. Res. 7: 1675.CrossRefGoogle Scholar
  53. 53.
    Seki, S. & Oda, T. (1980) Biochim. Biophys. Acta 606: 246.CrossRefGoogle Scholar
  54. 54.
    Castellot, J.J., Miller, M.R., Lehtomaki, D.M. & Pardee, A.B. (1979) J. Biol. Chem. 254: 6904.PubMedGoogle Scholar
  55. 55.
    Coetzee, M.L., Chou, R. & Ove, P. (1978) Cancer Res. 38: 3621.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Hans Krokan
    • 1
  1. 1.Institute of Medical BiologyUniversity of TromsøTromsøNorway

Personalised recommendations