Effects of Ultraviolet-Irradiation on DNA Ligase Activity of Human Fibroblasts from Normal and Xeroderma Pigmentosum Donors

  • S. Nocentini
  • M. Mezzina
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 40)


Ultraviolet (UV)-irradiation of stationary cultures induces an increase of DNA ligase activity in both normal and complementation group A xeroderma pigmentosum (XP) cells. Extents and doses of induction are cell line-dependent. These inductions can be prevented by the protein synthesis inhibitor cycloheximide, but not by the DNA replication inhibitor fluorodeoxyuridine (FudR).


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    S. Söderhäll and T. Lindahl, DNA ligases of eukaryotes, Febs Letters, 67: 1 (1976)CrossRefGoogle Scholar
  2. 2.
    M. Mezzina and S. Nocentini, DNA ligase activity in UV-irradiated monkey kidney cells, Nucleic Acids Res., 5: 4317 (1978)CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    J. E. Cleaver and J. E. Trosko, Absence of excision of ultraviolet-induced cyclobutane dimers in xeroderma pigmentosum, Photochem. Photobiol., 11: 547 (1970)CrossRefGoogle Scholar
  4. 4.
    M. C. Paterson, P. H. M. Lohman and M. L. Sluyter, Use of a UV endonuclease from Micrococcus luteus to monitor the progress of DNA repair in UV-irradiated human cells, Mutation Res., 19: 245 (1973)CrossRefPubMedGoogle Scholar
  5. 5.
    K. Tanaka, M. Sekiguchi and Y. Okada, Restoration of ultraviolet-induced unscheduled DNA synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage T4 endonuclease V and HVJ (Sendai virus), Proc. Nat. Acad. Sci. USA, 72: 4071 (1975)CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    A. J. Fornace, K. W. Kohn and H. E. Kann, DNA single-strands breaks during repair of UV damage in human fibroblasts and abnormalities of repair in xeroderma pigmentosum, Proc. Nat. Acad. Sci. USA, 73: 39 (1976)CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    P. R. Cook, I. A. Brazell, S. A. Pawsey and F. Giannelli, Changes induced by ultraviolet light in the superhelical DNA of lymphocytes from subjects with xeroderma pigmentosum and normal controls, J. Cell Sci., 29: 117 (1978)PubMedGoogle Scholar
  8. 8.
    C. W. Dingman and T. Kakunaga, DNA strand breaking and rejoining in response to ultraviolet light in normal and xeroderma pigmentosum cells, Int. J. Radiat. Biol., 30: 55 (1976)Google Scholar
  9. 9.
    J. E. Cleaver, Sedimentation of DNA from Human Fibroblasts Irradiated with Ultraviolet Light: Possible Detection of Excision Breaks in Normal and Repair-Deficient Xeroderma Pigmentosum Cells, Radiat. Res., 57: 207 (1974)Google Scholar
  10. 10.
    K. Cook, E. C. Friedberg and J. E. Cleaver, Excision of thymine dimers from specifically incised DNA by extracts of Xeroderma pigmentosum cells, Nature, Lond., 256: 235 (1975)CrossRefGoogle Scholar
  11. 11.
    K. Mortelmans, E. C. Friedberg, H. Slor, G. Thomas and J. E. Cleaver, Defective thymine dimer excision by cell-free extracts of xeroderma pigmentosum cells, Proc. Natl. Acad. Sci USA, 73: 2757 (1976)CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • S. Nocentini
    • 1
  • M. Mezzina
    • 2
  1. 1.Institut CurieSection de BiologieParis cedex 05France
  2. 2.Institut de Recherches Scientifiques sur le CancerCNRSVilleJuifFrance

Personalised recommendations