Cloning of the uvrC+ Gene from Escherichia coli Onto a Plaque Forming Phage Vector
Specialized transducing phages are important tools in the characterization of host coded gene functions. Isolation of specialized transducing phages was previously restricted to those carrying host genes located close to the normal phage attachment sitesl. However, recent advances in techniques of DNA recombination both in vivo and in vitro have made possible in principle the isolation of transducing phages carrying genes from anywhere on the bacterial chromosome2–4. This communication describes the isolation of a plaque-forming bacteriophage λ carrying the E.coli uvrC + gene.
KeywordsPhage Genome Host Bacterium Transduce Phage Phage Population Phage Vector
Unable to display preview. Download preview PDF.
- 1.M.E. Gottesman and R.A. Weisberg, in: “Bacteriophage Lambda”, A.D. Hershey, ed., Cold Spring Harbour Laboratory, New York (1971).Google Scholar
- 9.E. Seeberg, Mutat. Res. (in press).Google Scholar
- 13.R.W. Hendrix, in: “Bacteriophage Lambda”, A.D. Hershey, ed., Cold Spring Harbour Laboratory, New York (1971).Google Scholar
- 15.H. Pannekock, I.A. Noordermeer, C.A. van Sluis, and P. van de Putte, J. Bacteriol. 133: 884 (1978).Google Scholar
- 16.W.P. Rupp, A. Sancar, W.J. Kennedy, J. Ayers, and J. Griswold, in: “DNA Repair Mechanisms”, P.C. Hanawalt, E.C. Friedberg and C.F. Fox, eds., Academic Press, New York (1978)Google Scholar
- 17.C.A. van Sluis and R. Brandsma, this volume (1981).Google Scholar