Cloning of the uvrC+ Gene from Escherichia coli Onto a Plaque Forming Phage Vector

  • O. R. Blingsmo
  • A. L. Steinum
  • E. Rivedal
  • E. Seeberg
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 40)


Specialized transducing phages are important tools in the characterization of host coded gene functions. Isolation of specialized transducing phages was previously restricted to those carrying host genes located close to the normal phage attachment sitesl. However, recent advances in techniques of DNA recombination both in vivo and in vitro have made possible in principle the isolation of transducing phages carrying genes from anywhere on the bacterial chromosome2–4. This communication describes the isolation of a plaque-forming bacteriophage λ carrying the E.coli uvrC + gene.


Phage Genome Host Bacterium Transduce Phage Phage Population Phage Vector 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • O. R. Blingsmo
    • 1
  • A. L. Steinum
    • 1
  • E. Rivedal
    • 1
  • E. Seeberg
    • 1
  1. 1.Norwegian Defence Research EstablishmentKjellerNorway

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