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Plasmids Carrying the uvrA and uvrC Genes of Escherichia coli K12: Construction and Properties

  • Cees A. van Sluis
  • Jourica A. Brandsma
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 40)

Abstract

From the Carbon-Clarke collection, a plasmid harbouring the uvrC gene was identified (pLC13–12) and subsequently subcloned into pBR322. The UvrC+ plasmid pCA32 complemented UvrC deficient bacteria to the wild type phenotype. The uvrC gene has been assigned to a 2,000 bp BgIII DNA fragment. Upon introduction into minicells, pCA32 directs the synthesis of a protein of 28,000 dalton, which most likely is encoded by the uvrC gene.A novel method is described for the cloning of genes, for which direct selection is impracticable. The technique has been employed in the construction of a plasmid carrying lexA, uvrA, ssb on pACYC184.

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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Cees A. van Sluis
    • 1
  • Jourica A. Brandsma
    • 1
  1. 1.Laboratory of Molecular GeneticsState University of LeidenLeidenThe Netherlands

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