Cloning of recA+ and lexA+ and Some of Their Mutant Alleles; An Investigation of Their Mutual Interaction
The recA+, recA200 (ts), lexA+ and lexA3 alleles have been cloned into mn ttïcopy plasmid vectors. The plasmid pPE13 (recA+) confers only partial UV-resistance on DM49 (lexA3), despite producing an excess of recA protein. This suggests that lexA has another role in DNA repair in addition to its role in control of recA. A small degree of UV-sensitivity is conferred upon AB1157 (recA+ by pPE13 (recA+). At 42°, the plasmid pPE23 (recA200, ts) confers a high degree of UV-sensitivity upon AB1157 (recA+), despite the presence of the chromosomal recA+ gene. Purified recA200 (ts) protein at 420 has a single strand DNA-dependent ATPase activity which is higher than that of purified recA+ protein.
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