The Use of DNA Tumour Viruses as Model Systems for Studying Damage and Repair of Eukaryotic Chromosomes: Analysis of Mutations by DNA Sequencing

  • Beverly E. Griffin
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 40)


Attempts to define repair processes in eukaryotic systems are subject to all the difficulties inherent in studying very large and complex DNA molecules. When the location of a lesion that makes repair necessary for the efficient functioning of a cell cannot in itself be precisely defined, it is not surprising that the subsequent repair process can only be described in general terms, usually related to phenotypic responses. The idea that viruses might be used as probes for studying repair of DNA in mammalian cells has been put forward in a number of papers and a limited number of experiments have been carried out with this aim in mind. The conclusions from some of these studies, presented and summarised by Day (1978), do not make very encouraging reading. Nonetheless, it would seem premature to abandon the approach, particularly when one considers the remarkable advances made in the past few years in defining the molecular biology of some of the animal viruses and the promising efforts to correlate particular regions of viral genomes with biological activities inside the cell. For the purposes of this communication, I shall concentrate on the small DNA tumour viruses, the mouse virus, polyoma, the monkey virus, SV40, and the human variant of SV40, BKV as being among the better-characterised and most amenable to study of these viruses.


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  1. Bendig, M., Thomas, T. and Folk, W.R., 1980, J. Virol., 33: 1215.PubMedPubMedCentralGoogle Scholar
  2. Crémisi, C., 1979, in: “Microbiological Reviews”, p. 297.Google Scholar
  3. Cornelis, J.J., Lupker, J.H. and van der Eb, A.J., 1980, Mutation Res., 71: 139.CrossRefGoogle Scholar
  4. Das Gupta, U.B. and Summers, W.C., 1978, Proc. Nat. Acad. Sci. U.S.A., 75: 2378.Google Scholar
  5. Day, R.S., 1978, in: “DNA Repair Mechanisms”, Hanawalt, Friedberg and Fox, ed., ICN-UCLA Symp. on Molecular and Cellular Biology, I X: 531.Google Scholar
  6. Deininger, P.L., Esty, A., LaPorte, P., Hsu, H. and Friedmann, T., 1980, Nuc. Acids Res., 8: 855.Google Scholar
  7. DiMaio, D. and Nathans, D.J., 1980, J. Mol. Biol., 140: 129.CrossRefGoogle Scholar
  8. Fiers, W., Contreras, R., Haegeman, G., Rogiers, R., van de Voorde, A., van Heuverswyn, H., van Herreweghe, J., Volckaert, G. and Ysebaert, H., 1978, Nature, 273: 113.CrossRefGoogle Scholar
  9. Griffin, B.E. and Maddock, C., 1979, J. Virol., 31: 645.PubMedPubMedCentralGoogle Scholar
  10. Hale, P., Woodward, R.S. and Lebowitz, J., 1980, Nature, 284:640.CrossRefGoogle Scholar
  11. Hattori, J., Carmichael, G.G. and Benjamin, T.L., 1979, Cell, 16: 505.CrossRefGoogle Scholar
  12. Ito, Y., Brocklehurst, J.R. and Dulbecco, R., 1977, Proc. Nat. Acad. Sci. U.S.A., 74: 4666.CrossRefGoogle Scholar
  13. Ito, Y., Spurr, N. and Griffin, B.E., J. Virol., in press.Google Scholar
  14. Jelinek, W.R., Toomey, T.P., Leinwand, L., Duncan, C.H., Brio, P.A., Choudary, P.V., Weissman, S.M., Rubin, C.M., Houck, C.M., Deininger, P.L. and Schmid, C.W., 1980, Proc. Nat. Acad. Sci. U.S.A., 77: 1398.CrossRefGoogle Scholar
  15. Katinka, M., Yaniv, M., Vasseur, M. and Blangy, D., 1980, Cell, 20: 393.CrossRefGoogle Scholar
  16. Lai, C.-J. and Nathans, D., 1975, Virol., 66: 70.CrossRefGoogle Scholar
  17. Lim, V.I. and Mazanov, A.L., 1978, FEBS Letters, 88: 118.CrossRefGoogle Scholar
  18. Maxam, A.M. and Gilbert, W., 1977, Proc. Nat. Acad. Sci. U.S.A., 74: 560.CrossRefGoogle Scholar
  19. Miller, L.K., Cooke, B.E. and Fried, M., 1976, Proc. Nat. Acad. Sci. U.S.A., 73: 3070.Google Scholar
  20. Novak, U., Dilworth, S.M. and Griffin, B.E., 1980, Proc. Nat. Acad. Sci. U.S.A., 77: 3278.CrossRefGoogle Scholar
  21. Reddy, V.B., Thimmapaya, B., Dhar, R., Subramanian, K.N., Zain, B.S., Pan, J., Ghosh, P.K., Celma, M.L. and Weissman, S.M., 1978, Science, 200: 494.CrossRefGoogle Scholar
  22. Sanger, F., Nicklen, S. and Coulson, A.R., 1977, Proc. Nat. Acad. Sci. U.S.A., 74: 5463.CrossRefGoogle Scholar
  23. Seif, I., Khoury, G. and Dhar, R., 1980, Nuc. Acids Res., 8:2225.CrossRefGoogle Scholar
  24. Shortle, D.R., Margolskee, R.F. and Nathans, D., 1979, Proc. Nat. Acad. Sci. U.S.A., 76: 6128.CrossRefGoogle Scholar
  25. Soeda, E. and Griffin, B.E., 1978, Nature, 276: 294.CrossRefGoogle Scholar
  26. Soeda, E., Arrand, J.R., Smolar, N. and Griffin, B.E., 1979, Cell, 17: 357.CrossRefGoogle Scholar
  27. Soeda, E., Arrand, J.R., Smolar, N., Walsh, J.E. and Griffin, B.E., 1980, Nature, 283: 445.CrossRefGoogle Scholar
  28. Tjian, R., 1978, Cell, 13: 165.CrossRefGoogle Scholar
  29. Tooze, J. (ed.), 1980, “Molecular Biology of Tumor Viruses Part 2: DNA Tumor Viruses”, Cold Spring Harbor Lab.Google Scholar
  30. Yang, R.C. and Wu, R., 1979, Science, 206: 456.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Beverly E. Griffin
    • 1
  1. 1.Imperial Cancer Research FundLincoln’s Inn FieldsLondon W.C. 2England

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