Isolation of Mutants of Escherichia coli with Increased Resistance to Alkylating Agents

  • Barbara Sedgwick
  • Peter Robins
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 40)


A search was made for strains constitutive for the adaptive response by isolating mutants with increased resistance to killing by methylating agents. N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) resistant strains were obtained, but these proved to be less readily methylated by MNNG because they had a low intracellular thiol content (thiols convert MNNG to the proximal mutagen, methylnitrosamine (5)). Four strains with increased resistance to N-methylnitrosourea (MNU) proved to be constitutive for the adaptive response. After exposure to (3H) MNNG, they showed the same low ratio of O6-methylguanine (O6MeG) to 7-methylguanine (N7MeG) as adapted wild type bacteria. When exposed to very high MNNG doses, they were even more resistant to killing and mutation than the adapted wild type. The constitutives may therefore have an even greater ability than the adapted wild type to repair O6MeG.


Adaptive Response Resistant Mutant ALKYLATING Agent Wild Type Bacterium Cellular Thiol 
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  1. 1.
    Apontoweil, P. and Berends, W. (1975) Biochim. Biophys. Acta 399, 1CrossRefGoogle Scholar
  2. 2.
    Jeggo, P., Defais, M., Samson, L. and Schendel P. (1977) Mòlec. gen. Genet., 157, 1.PubMedCrossRefGoogle Scholar
  3. 3.
    Jeggo, P. (1979) J. Bact. 139, 783.PubMedGoogle Scholar
  4. 4.
    Karran, P., Lindahl, T., and Griffin, B. (1979) Nature (lond.) 280, 76.CrossRefGoogle Scholar
  5. 5.
    Lawley, P.D. and Thatcher, C.J. (1970) Biochem. J. 116, 693.PubMedGoogle Scholar
  6. 6.
    Samson, L. and Cairns, J. (1977) Nature (Lond.) 267, 281.CrossRefGoogle Scholar
  7. 7.
    Schendel, P.F. and Robins, P. (1978) Proc. Natl. Acad. Sci., (USA) 75, 6017.CrossRefGoogle Scholar
  8. 8.
    Wheeler, G.P. and Bowdon, B.J. (1972) Biochem. Pharmacol., 21, 265.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Barbara Sedgwick
    • 1
  • Peter Robins
    • 1
  1. 1.Mill Hill LaboratoriesImperial Cancer Research FundLondonEngland

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