Repair of UV-Damaged DNA Transformed into E. coli
The ability of repair proficient and deficient strains of E. coli to repair transforming plasmid DNA has been investigated, using calcium treatment to render the cells competent. Excision repair pathways are particularly important for transforming DNA, but recA mediated pathways play only a minor role. The presence of a homologous resident plasmid in the recipient cell is shown to enhance the survival of incoming damaged plasmid. This enhancement depends on the host uvrA+ and uvrB+ gene products, but not on host recA functions. Explanations of the enhanced survival are considered, based on the known interactions of plasmid molecules.
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