The Use of Direct Photoreversal of UV-Irradiated DNA for the Demonstration of Pyrimidine Dimer-DNA Glycosylase Activity

  • Eric H. Radany
  • Jack D. Love
  • Errol C. Friedberg
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 40)


A two-step enzymatic mechanism for the incision of UV-irradiated DNA at pyrimidine dim ers (PD) by the “UV endonuclease” activity from M. luteus has recently been proposed (1–3). This mechanism is related to the classical base excision repair mode (4), since it consists of the sequential action of a PD-specific DNA glycosylase and an apurinic/apyrimidinic (AP) endonuclease. Such a two-step mechanism was interesting to us for the relevance it might have to our continuing investigations of the repair of UV-irradiated DNA in a variety of biological systems.


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  1. 1.
    Grossman, L., Riazzudin, S., Haseltine, W.A., and Lindan, C. (1979) Cold Spring Harb. Symp. Quant. Biol. 43, 947.CrossRefGoogle Scholar
  2. 2.
    Haseltine, W.A., Gordon, L.K., Lindan, C.P., Grafstrom, R.H., Shaper, N.L., and Grossman, L. (1980) Nature 285, 634.CrossRefGoogle Scholar
  3. 3.
    Grafstrom, R., Shaper, N., Grossman, L., and Haseltine, W.A. (1981) (this volume).Google Scholar
  4. 4.
    Friedberg, E.C. (this volume).Google Scholar
  5. 5.
    Radany, E.H., and Friedberg, E.C. (1980) Nature 286, 182.CrossRefGoogle Scholar
  6. 6.
    Friedberg, E.G., Anderson, C.T.M., Bonura, T., Cone, R., Radany, E.H., and Reynolds, R.J. (1980). Prow. Nuc. Acid Res. Mol. Biol. 26, 197.CrossRefGoogle Scholar
  7. 7.
    Setlow, R.B. (1966) Science 153, 379.CrossRefGoogle Scholar
  8. 8.
    Setlow, R.B. (1968) Frog. Nuc. Acid Res. Mol. Biol. 8, 257.CrossRefGoogle Scholar
  9. 9.
    Rahn, R.O. and Landry, L.C. (1973) Photochem. Photobiol. 18, 29.CrossRefGoogle Scholar
  10. 10.
    Yasuda, S., and Sekiguchi, M. (1970) Proc. Natl. Acad. Sci. (US) 67, 1839.CrossRefGoogle Scholar
  11. 11.
    Friedberg, E.C., and Lehman, I.R. (1974) Biochem. Biophys. Res. Comm 58, 132.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Eric H. Radany
    • 1
  • Jack D. Love
    • 1
  • Errol C. Friedberg
    • 1
  1. 1.Laboratory of Experimental Oncology Department of PathologyStanford UniversityStanfordUSA

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