Incision of Pyrimidine Dimer Containing DNA by Small Molecular Weight Enzymes

  • R. Grafstrom
  • N. Shaper
  • L. Grossman
  • W. Haseltine
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 40)


In Micrococcus luteus the incision event during repair of DNA containing pyrimidine dimers has been shown in vitro to be a two-step enzymatic reaction. The first step results in the cleavage of the N-glycosylic bond between the 5′-thymine moiety of the pyrimidine dimer and the deoxyribose generating an apyrimidinic site (Apy) and a thymine-thymidylate dimer attached to the DNA. The second catalytic step involves phosphodiester bond hydrolysis 3′ to the Apy site generating a nicked DNA with an Apy site at its 3′ terminus and the mixed thymine-thymidylate dimer at the 5′ terminus.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. (1).
    Riazuddin, S. and Grossman, L., J. Biol. Chem 252, 6280 (1977).PubMedGoogle Scholar
  2. (2).
    Grossman, L, Riazuddin, S., Haseltine, W.A. and Lindan, C., Cold Spring Harbor Symp. on Quant. Biol. 43, 947 (1979).Google Scholar
  3. (3).
    Haseltine, W.A., Lindan, C.P., D’Andrea, A.D. and Johnsrud, L., Meth. in Enzym. 65, 235 (1980).CrossRefGoogle Scholar
  4. (4).
    Haseltine, W.A., Gordon, L.K., Lindan, C.P., Grafstrom, R.H., Shaper, N.L. and Grossman, L., Nature, 285, 634 (1980).CrossRefGoogle Scholar
  5. (5).
    Maxam, A and Gilbert, W., Proc. Natl. Acad. Sci. 74, 560 (1977).CrossRefGoogle Scholar
  6. (6).
    Shaper, N.L. and Grossman, L., Meth. in Enzym. 65, 216 (1980).CrossRefGoogle Scholar
  7. (7).
    Weiss, B., J. Biol. Chem. 251, 1896 (1976).PubMedGoogle Scholar
  8. (8).
    Hamilton, L.D.G., Mahler, I. and Grossman, L., Biochem. 13, 1886 (1974).CrossRefGoogle Scholar
  9. (9).
    Kaplan, J.C., Kushner, S.R. and Grossman, L., Biochem. 10, 3315 (1971).CrossRefGoogle Scholar
  10. (10).
    Chase, J. and Richardson, C.C., J. Biol. Chem. 249, 4553 (1974).PubMedGoogle Scholar
  11. (11).
    Haseltine, W.A., personal communication.Google Scholar
  12. (12).
    Friedberg, E., privileged communication.Google Scholar
  13. (13).
    Yeung, G.T., unpublished data.Google Scholar
  14. (14).
    Seeberg, E., Proc. Natl. Acad. Sci. 75, 2569 (1978).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • R. Grafstrom
    • 1
  • N. Shaper
    • 1
  • L. Grossman
    • 1
  • W. Haseltine
    • 2
  1. 1.Department of BiochemistryThe Johns Hopkins University School of Hygiene and Public HealthBaltimoreUSA
  2. 2.Sidney Farber Cancer InstituteHarvard UniversityUSA

Personalised recommendations