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Incision of Pyrimidine Dimer Containing DNA by Small Molecular Weight Enzymes

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Chromosome Damage and Repair

Part of the book series: NATO Advanced Study Institutes Series ((NSSA,volume 40))

Abstract

In Micrococcus luteus the incision event during repair of DNA containing pyrimidine dimers has been shown in vitro to be a two-step enzymatic reaction. The first step results in the cleavage of the N-glycosylic bond between the 5′-thymine moiety of the pyrimidine dimer and the deoxyribose generating an apyrimidinic site (Apy) and a thymine-thymidylate dimer attached to the DNA. The second catalytic step involves phosphodiester bond hydrolysis 3′ to the Apy site generating a nicked DNA with an Apy site at its 3′ terminus and the mixed thymine-thymidylate dimer at the 5′ terminus.

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Bibliography

  1. Riazuddin, S. and Grossman, L., J. Biol. Chem 252, 6280 (1977).

    PubMed  CAS  Google Scholar 

  2. Grossman, L, Riazuddin, S., Haseltine, W.A. and Lindan, C., Cold Spring Harbor Symp. on Quant. Biol. 43, 947 (1979).

    CAS  Google Scholar 

  3. Haseltine, W.A., Lindan, C.P., D’Andrea, A.D. and Johnsrud, L., Meth. in Enzym. 65, 235 (1980).

    Article  CAS  Google Scholar 

  4. Haseltine, W.A., Gordon, L.K., Lindan, C.P., Grafstrom, R.H., Shaper, N.L. and Grossman, L., Nature, 285, 634 (1980).

    Article  CAS  PubMed  Google Scholar 

  5. Maxam, A and Gilbert, W., Proc. Natl. Acad. Sci. 74, 560 (1977).

    Article  CAS  PubMed  Google Scholar 

  6. Shaper, N.L. and Grossman, L., Meth. in Enzym. 65, 216 (1980).

    Article  CAS  Google Scholar 

  7. Weiss, B., J. Biol. Chem. 251, 1896 (1976).

    PubMed  CAS  Google Scholar 

  8. Hamilton, L.D.G., Mahler, I. and Grossman, L., Biochem. 13, 1886 (1974).

    Article  CAS  Google Scholar 

  9. Kaplan, J.C., Kushner, S.R. and Grossman, L., Biochem. 10, 3315 (1971).

    Article  CAS  Google Scholar 

  10. Chase, J. and Richardson, C.C., J. Biol. Chem. 249, 4553 (1974).

    PubMed  CAS  Google Scholar 

  11. Haseltine, W.A., personal communication.

    Google Scholar 

  12. Friedberg, E., privileged communication.

    Google Scholar 

  13. Yeung, G.T., unpublished data.

    Google Scholar 

  14. Seeberg, E., Proc. Natl. Acad. Sci. 75, 2569 (1978).

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biochemistry, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland, 21205, USA

    R. Grafstrom, N. Shaper & L. Grossman

  2. Sidney Farber Cancer Institute, Harvard University, USA

    W. Haseltine

Authors
  1. R. Grafstrom
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  2. N. Shaper
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  3. L. Grossman
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  4. W. Haseltine
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Editor information

Editors and Affiliations

  1. Norwegian Defense Research Establishment, Kjeller, Norway

    Erling Seeberg

  2. University of Bergen, Bergen, Norway

    Kjell Kleppe

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© 1981 Plenum Press, New York

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Grafstrom, R., Shaper, N., Grossman, L., Haseltine, W. (1981). Incision of Pyrimidine Dimer Containing DNA by Small Molecular Weight Enzymes. In: Seeberg, E., Kleppe, K. (eds) Chromosome Damage and Repair. NATO Advanced Study Institutes Series, vol 40. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-7956-0_11

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  • DOI: https://doi.org/10.1007/978-1-4684-7956-0_11

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4684-7958-4

  • Online ISBN: 978-1-4684-7956-0

  • eBook Packages: Springer Book Archive

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