A Competitive Binding Protein Assay for Juvenile Hormone

  • W. Goodman
  • W. E. Bollenbacher
  • H. L. Zvenko
  • L. I. Gilbert


Despite the discovery of juvenile hormone (JH) four decades ago (Wigglesworth, 1934) and the structural identification of the molecule nearly a decade ago (Röller et al., 1967), the lack of a simple, rapid, and sensitive quantification procedure for JH has hindered progress in the field of insect endocrinology. The minute quantities of hormone, the paucity of insect tissue and the high potential for degradation contribute to the technical difficulties encountered when titering JH. At present, two methods are being used to determine JH titers, analytical chromatography (van Broekhoven et al. 1975; Dahm et al.this volume; Schooley et al., this volume) and bioassay (Staal, 1972; Bjerke and Röller, 1974).


Juvenile Hormone Hexane Extract Fourth Instar Juvenile Hormone Titer Mixed Isomer 
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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • W. Goodman
    • 1
  • W. E. Bollenbacher
    • 1
  • H. L. Zvenko
    • 1
  • L. I. Gilbert
    • 1
  1. 1.Department of Biological SciencesNorthwestern UniversityEvanstonUSA

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