The Effects of Juvenile Hormone on Imaginal Discs of Drosophila In Vitro: The Role of the Inhibition of Protein Synthesis

  • J. W. Fristrom
  • C. J. Chihara
  • L. Kelly
  • J. T. Nishiura


We have been studying the genetic, molecular and developmental processes involved in the evagination of imaginal discs of Drosophila melanogaster. These studies have been facilitated, on the one hand, by the fact that evagination occurs in vitro in a variety of culture media in response to ecdysones (Mandaron, 1973; Chihara et al., 1972; Fristrom et al., 1973; Milner and Sang, 1974), and, on the other hand, by the availability of large numbers of imaginal discs (600,000–800,000 per day) isolated by a preparative procedure (Fristrom, 1972). Thus we have the advantage of both defined in vitro conditions and sufficient material for biochemical analysis. The morphology of an unevaginated and fully evaginated leg disc is compared in Fig. 1. Ecdysone-induced evagination is inhibited by all three of the known, naturally occurring juvenile hormones (JHs) and some, but not all, juvenile hormone analogs (Siegel and Fristrom, in press). In addition to inhibiting evagination, juvenile hormone also inhibits, under some conditions, DNA synthesis (Logan et al., 1975), RNA synthesis (Chihara and Fristrom, 1973) and protein synthesis, and affects some membrane properties (e.g., uridine transport, Chihara and Fristrom, 1973).


Protein Synthesis Juvenile Hormone Imaginal Disc Disc Cell Ouabain Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ashburner, M., Chihara, C., Meltzer, P., and Richards, G., 1973, Cold Spring Harbor Symp. Quant. Biol. 38: 655.CrossRefGoogle Scholar
  2. Chihara, C., and Fristrom, J., 1973, Develop. Biol. 35: 36.PubMedCrossRefGoogle Scholar
  3. Chihara, C., Petri, W., Fristrom, J., and King, D., 1972, J. Insect Physiol. 18: 1115.PubMedCrossRefGoogle Scholar
  4. Cohen, E., Edelman, Emmerich, Fekete, E., Wilhelm and Gilbert, L., 1973, J. Insect Physiol. 19: 1857.PubMedCrossRefGoogle Scholar
  5. Edelman, I.S., 1975, J. Steroid Biochem. 6: 147.PubMedCrossRefGoogle Scholar
  6. Emmerich, H., 1970, Z. vergl. Physiol. 68: 385.CrossRefGoogle Scholar
  7. Fekete, E., Fristrom, D., Kiss, I., and Fristrom, J., 1975, Wilhelm Roux’ Arch. Entwicklungsmech. Organ. 178: 123.Google Scholar
  8. Fristrom, J., 1972, in “The Biology of Imaginal Discs” (H. Ursprung Nöthiger, eds.) pp. 109–154, Springer-Verlag, Berlin.Google Scholar
  9. Fristrom, J., Logan, W., and Murphy, C., 1973, Develop. Biol. 33: 441.PubMedCrossRefGoogle Scholar
  10. Fristrom, J., Gregg, T., and Siegel, J., 1974, Develop. Biol. 41: 301.PubMedCrossRefGoogle Scholar
  11. Kroeger, H., 1963, Nature 200:1234. 1973, Exp. Cell Res. 80: 329.Google Scholar
  12. Lezzi, M., and Gilbert, L., 1972, Gen. Comp. Endocrinol. Suppl. 3: 159.Google Scholar
  13. Logan, W., Fristrom, D., and Fristrom, J., 1975, J. Insect Physiol. 21: 1343.PubMedCrossRefGoogle Scholar
  14. Mandaron, P., 1973, Develop. Biol. 31: 101.PubMedCrossRefGoogle Scholar
  15. Milner, M., and Sang, J., 1974, Cell 3: 141.PubMedCrossRefGoogle Scholar
  16. Nishiura, J., and Fristrom, J., 1975, Proc. Nat. Acad. Sci., USA 72: 2984.CrossRefGoogle Scholar
  17. Poodry, C., and Schneiderman, H., 1971, Wilhelm Roux’ Arch. Entwicklungsmech. Organ. 168: 1.CrossRefGoogle Scholar
  18. Robb, J., 1969, J. Cell Biol. 41: 876.PubMedCrossRefGoogle Scholar
  19. Siegel, J., and Fristrom, J., 1976, in“Biology of Drosophila,” (M. Ashburner and T. Wright, eds.) v. II, Academic Press, New York, in press.Google Scholar
  20. Yund, M., and Fristrom, J., 1975a, Develop. Biol. 43: 287.PubMedCrossRefGoogle Scholar
  21. Yund, M., and Fristrom, J., 1975b, Proc. ICN, UCLA Squaw Valley Conf. Develop. Biol., in press.Google Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • J. W. Fristrom
    • 1
  • C. J. Chihara
    • 1
  • L. Kelly
    • 1
  • J. T. Nishiura
    • 1
  1. 1.Department of GeneticsUniversity of CaliforniaBerkeleyUSA

Personalised recommendations