Quantitative Analysis of Eicosanoids by Gas Chromatography-Mass Spectrometry
Eicosanoids are metabolic products derived from polyunsaturated straight-chain C 20 carboxylic acids. The most abundant substrate in humans is arachidonic acid (AA), a physiological component of the plasma membrane. Following stimulation, AA is released from an ester linkage to phospholipids and oxygenated into an array of compounds whose biological importance has been well established in vitro. On the other hand, the investigation of their potential role in human pathology depends upon assessment of their formation in vivo. Alterations in eicosanoid biosynthesis in pathological conditions and the functional consequences of their pharmacological inhibition or antagonism have indicated their pathophysiological role in vivo1–5. Specific and sensitive assays for eicosanoids have been required to address these issues.
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