Gene Expression during Growth and Maturation
The expression of cab and rbcS gene families were measured in RNA extracted from needles from larch trees ranging in age from 1 y to 75 y. Steady state cab mRNA levels are relatively higher (~40%) in newly expanding short shoot foliage from juvenile plants compared with mature plants. Later in the season no consistent difference in cab expression between juvenile and mature plants was detected. Unlike cab gene expression, the expression of the rbcS gene family did not seem to vary with age. These data show that the maturation-related changes in morphological and physiological phenotypes are associated with changes in gene expression. No causal relationship has been established, however. Indeed, we conclude that the faster growth of juvenile scions is not due to increased net photosynthesis or cab expression.
Unable to display preview. Download preview PDF.
- 4.Cheliak W. M. and Pitel, J. A., 1985, Inheritance and linkage of allozymes in Larix laricina, Silvae Gen. 34: 142–148.Google Scholar
- 6.Dhillon S. S., 1987, DNA in tree species, in: “Cell and Tissue Culture in Forestry: General Principles and Biotechnology,” J. M. Bonga and D. J. Durzan. eds. Martinous Nijhoff, Boston. pp. 293–313.Google Scholar
- 7.Durzan, D. J., 1984, Special problems: adult vs. juvenile expiants, in: “Handbook of plant cell culture” v2, Sharp, Ammirato, Yanada, eds., MacMillan Pub. Co..Google Scholar
- 12.Greenwood M. S. and Hutchison, K. W., Maturation as a developmental process, in: “Clonal Forestry: Genetics, Biotechnology and Application.” M. R. Ahuja and W. J. Libby, eds., Springer Verlag, New York (in press). Google Scholar
- 13.Hackett, W. P., 1985, Juvenility, maturation and rejuvenation in woody plants, Hort. Reviews 7: 109–155.Google Scholar
- 14.Hackett, W. P., Murray, J. and Woo, H., 1991, Biochemical and molecular analysis of maturation related characteristics in Hedera helix, in: “Woody Plant Biotechnology,” M. R. Ahuja, ed. Plenum, NY.Google Scholar
- 15.Hunyh, T. V., Young, R. A. and Davis, R. W., 1985, Constructing and screening cDNA libraries in lambda-GT10 and lambda-GT11. In: “DNA Cloning: A Practical Approach,” D. Glover, ed., IRL Press, Oxford, pp.49–78.Google Scholar
- 16.Hutchison, K., Greenwood, M., Sherman, C., Rebbeck, J. and Singer, P., 1990, The molecular genetics of maturation in eastern larch (Larix laricina [Du Roi] K. Koch), in: “Molecular basis of plant ageing.” R. Rodriguez, ed., Pergamon Press pp. 141–145.Google Scholar
- 17.Hutchison, K. W., Singer, P. B. and Greenwood, M. S., 1988, Molecular analysis of gene expression during the development and maturation of larch, in: W. M. Cheliak and A. C. Yapa (eds), “Molecular Genetics of Forest Trees. IUFRO Molecular Genetics Workshop.” Petawawa National Forestry Institute, Chalk River, Ont. Information Report PI-X-80, pp. 26–33.Google Scholar