Advertisement

Gene Expression during Growth and Maturation

  • Keith W. Hutchison
  • Patricia B. Singer
  • Michael S. Greenwood
Part of the NATO ASI Series book series (NSSA, volume 210)

Abstract

The expression of cab and rbcS gene families were measured in RNA extracted from needles from larch trees ranging in age from 1 y to 75 y. Steady state cab mRNA levels are relatively higher (~40%) in newly expanding short shoot foliage from juvenile plants compared with mature plants. Later in the season no consistent difference in cab expression between juvenile and mature plants was detected. Unlike cab gene expression, the expression of the rbcS gene family did not seem to vary with age. These data show that the maturation-related changes in morphological and physiological phenotypes are associated with changes in gene expression. No causal relationship has been established, however. Indeed, we conclude that the faster growth of juvenile scions is not due to increased net photosynthesis or cab expression.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Anderson, J. M., 1986, Photoregulation of the composition, function, and structure of thylakoid membranes. Annu. Rev. Plant Physiol. 37: 93–136.CrossRefGoogle Scholar
  2. 2.
    Bauer H. and Bauer, U., 1980, Photosynthesis in leaves of the juvenile and adult phase of ivy (Hedera helix), Physiol Plant. 49: 366–372.CrossRefGoogle Scholar
  3. 3.
    Borchert, R., 1976, Differences in shoot growth patterns between juvenile and adult trees and their interpretation based on systems analysis of trees. Acta Hortic. 56: 123–130.CrossRefGoogle Scholar
  4. 4.
    Cheliak W. M. and Pitel, J. A., 1985, Inheritance and linkage of allozymes in Larix laricina, Silvae Gen. 34: 142–148.Google Scholar
  5. 5.
    Denhardt, D. T., 1966, A membrane-filter technique for the detection of complementary DNA, Biochem. Biophys. Res. Commun. 23:641–646.CrossRefGoogle Scholar
  6. 6.
    Dhillon S. S., 1987, DNA in tree species, in: “Cell and Tissue Culture in Forestry: General Principles and Biotechnology,” J. M. Bonga and D. J. Durzan. eds. Martinous Nijhoff, Boston. pp. 293–313.Google Scholar
  7. 7.
    Durzan, D. J., 1984, Special problems: adult vs. juvenile expiants, in: “Handbook of plant cell culture” v2, Sharp, Ammirato, Yanada, eds., MacMillan Pub. Co..Google Scholar
  8. 8.
    Fins L. and Seeb, L. W., 1986, Genetic variation in allozymes of Western larch, Can. J. For. Res. 16: 1013–1018.CrossRefGoogle Scholar
  9. 9.
    Greenwood, M. S. Phase change in loblolly pine: shoot development as a function of age, Physiol Plant. 61: 518–522.CrossRefGoogle Scholar
  10. 10.
    Greenwood, M. S., 1987, Rejuvenation of forest trees, Plant Growth Reg. 6: 1–12.CrossRefGoogle Scholar
  11. 11.
    Greenwood, M. S., Hopper, C. A. and Hutchison, K. W., 1989, Maturation in larch. I. Effect of age on shoot growth, foliar characteristics, and DNA methylation. Plant Physiol. 90: 406–412.CrossRefGoogle Scholar
  12. 12.
    Greenwood M. S. and Hutchison, K. W., Maturation as a developmental process, in: “Clonal Forestry: Genetics, Biotechnology and Application.” M. R. Ahuja and W. J. Libby, eds., Springer Verlag, New York (in press). Google Scholar
  13. 13.
    Hackett, W. P., 1985, Juvenility, maturation and rejuvenation in woody plants, Hort. Reviews 7: 109–155.Google Scholar
  14. 14.
    Hackett, W. P., Murray, J. and Woo, H., 1991, Biochemical and molecular analysis of maturation related characteristics in Hedera helix, in: “Woody Plant Biotechnology,” M. R. Ahuja, ed. Plenum, NY.Google Scholar
  15. 15.
    Hunyh, T. V., Young, R. A. and Davis, R. W., 1985, Constructing and screening cDNA libraries in lambda-GT10 and lambda-GT11. In: “DNA Cloning: A Practical Approach,” D. Glover, ed., IRL Press, Oxford, pp.49–78.Google Scholar
  16. 16.
    Hutchison, K., Greenwood, M., Sherman, C., Rebbeck, J. and Singer, P., 1990, The molecular genetics of maturation in eastern larch (Larix laricina [Du Roi] K. Koch), in: “Molecular basis of plant ageing.” R. Rodriguez, ed., Pergamon Press pp. 141–145.Google Scholar
  17. 17.
    Hutchison, K. W., Singer, P. B. and Greenwood, M. S., 1988, Molecular analysis of gene expression during the development and maturation of larch, in: W. M. Cheliak and A. C. Yapa (eds), “Molecular Genetics of Forest Trees. IUFRO Molecular Genetics Workshop.” Petawawa National Forestry Institute, Chalk River, Ont. Information Report PI-X-80, pp. 26–33.Google Scholar
  18. 18.
    Whitmore F. W. and Kreibel, H. B., 1987, Expression of a gene in Pinus strobus ovules associated with fertilization and early embryo development, Can. J. For. Res. 17:408–412.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Keith W. Hutchison
    • 1
  • Patricia B. Singer
    • 1
  • Michael S. Greenwood
    • 2
  1. 1.Department of BiochemistryUniversity of MaineOronoUSA
  2. 2.Department of Forest BiologyUniversity of MaineOronoUSA

Personalised recommendations