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Somaclonal Variation in Populus Hybrids Regenerated from Protoplast Culture

  • Rod Serres
  • Mike Ostry
  • Brent McCown
  • Darroll Skilling
Part of the NATO ASI Series book series (NSSA, volume 210)

Abstract

Protoclones were regenerated from the poplar hybrid NC5339 (Populus alba × P. grandidentata ‘Crandon’). Morphologic variation primarily in leaf characteristics has been observed in four vegetative generations under both greenhouse and field conditions. Three variant tree types have been identified: (1) Normal growth rate with long leaves that have reduced pubescence on the underside of the leaves. Leaves show sectoring of pubescence levels. (2) Slower growing with thick, dark green, heavily pubescent leaves in highly convoluted, brittle leaves. (3) Dwarf fast-growing tree with ovate, pale green leaves and a tendency for heavy branching. Leaves show sectoring of pubescence levels. Possible explanations for the variation observed are offered.

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References

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    Larkin, P.J, and Scowcroft, W.R., 1981, Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor. Appi. Genet. 60:197–214.CrossRefGoogle Scholar
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    USDA (ed) Forest Service, 1980, Energy and wood from intensively cultured plantations; research and development program. General Technical Report NC-28.Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Rod Serres
    • 1
  • Mike Ostry
    • 2
  • Brent McCown
    • 1
  • Darroll Skilling
    • 2
  1. 1.Department of HorticultureUniversity of Wisconsin-MadisonSt. PaulUSA
  2. 2.North Central Forest Experiment StationUSDA-Forest ServiceSt. PaulUSA

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