Optimizing Potential for Adventitious Shoot Organogenesis in Hybrid Populus Explants In Vitro with Wound Treatment and Micro-Cross Sections

  • Ok Young Lee-Stadelmann
  • Seungwoo Lee
  • Haejoon Chung
  • Quansheng Guo
  • Myungwon Kim
  • Chunho Pak
  • Wesley P. Hackett
Part of the NATO ASI Series book series (NSSA, volume 210)


In vitro adventitious bud formation was investigated in 3 hybrid Populus clones with varying degrees of regeneration potential. Longitudinal and transverse wounding treatment of 1.0 cm leaf midvein and petiole expiants both increased the shoot regneration capacity, more effectively with transverse wounding. Use of micro-cross sections (400 um in thickness) of leaf midvein or stem internode greatly improved regeneration capacity in all 3 clones. Supplementary calcium nitrate (7.05 to 9.4 mM) with optimal BA and NAA concentrations enhanced regeneration potential of micro- cross sections of 2 clones, but not of the most recalcitrant clone. Micro-cross section are excellent expiant for obtaining large numbers of uniform adventitious shoots from minimum amount of material in a short regeneration time.


Adventitious Shoot Shoot Formation Naphthalene Acetic Acid Woody Plant Medium Petiole Explants 


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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Ok Young Lee-Stadelmann
    • 1
  • Seungwoo Lee
    • 2
  • Haejoon Chung
    • 3
  • Quansheng Guo
    • 4
  • Myungwon Kim
    • 5
  • Chunho Pak
    • 6
  • Wesley P. Hackett
    • 1
  1. 1.Department of Horticultural SciencesUniversity of MinnesotaSt. PaulUSA
  2. 2.Dept. of Hort.Kyung Hee Univ.SuwonKorea
  3. 3.Dept. of Hort.Pai-Chai Univ.DaejonKorea
  4. 4.Div. of Basic CoursesZhejiang Agr. Univ.Hangzhou, ZhejiangP.R. China
  5. 5.Dept. of Biol.Yonsei Univ.KangwondoKorea
  6. 6.Dept. of Hort. BreedingMokpo National Univ.ChonnamKorea

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