Cryopreservation of Haploid Embryos of Quercus Petraea, Fagus Sylvatica and Somatic Embryos of Aesculus Hippocastanum
Globular or heart-shaped haploid embryos of oak and beech and somatic horse-chestnut embryos in the same stage were deep-frozen with a modified method of SAKAI. After a few days the cryopreserved embryos were rewarmed and placed on modified MS-media or WPM. After one month the embryos grew in the same manner as unfrozen embryos.
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