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Cryopreservation of Haploid Embryos of Quercus Petraea, Fagus Sylvatica and Somatic Embryos of Aesculus Hippocastanum

  • Jörg Jörgensen
Part of the NATO ASI Series book series (NSSA, volume 210)

Abstract

Globular or heart-shaped haploid embryos of oak and beech and somatic horse-chestnut embryos in the same stage were deep-frozen with a modified method of SAKAI. After a few days the cryopreserved embryos were rewarmed and placed on modified MS-media or WPM. After one month the embryos grew in the same manner as unfrozen embryos.

References

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    JÖRGENSEN, J. (1988) Embryogenesis in Ouercus petraea and Fagus sylvatica. J. Plant. Physiol. 132, 638–640.CrossRefGoogle Scholar
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    JÖRGENSEN, J. (1989) Somatic embryogenesis in Aesculus hippocastanum L. by culture of filament callus, J. Plant. Physiol, in press.CrossRefGoogle Scholar
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    LLOYD, G. AND MCCOWN, B. H. (1981) Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot-tip culture, Proc. Int. Plant Prop. Soc, 30, 421–427.Google Scholar
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    SAKAI, A. (1985) Cryoprservation of shoot-tips of fruit trees and herbaceous plants, In: Cryopreservation of Plant Cells and Organs (Kartha, K.K. ed.) CRC Press, Inc., 135–158.Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Jörg Jörgensen
    • 1
  1. 1.Niedersächsische Forstliche VersuchsanstaltStaufenberg-EscherodeGermany

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