Somatic Embryogenesis in Aesculus Hippocastanum and Quercus Petraea from Old Trees (10 to 140 Years)
Tissues of the flower of oak (anthers) and horse-chestnut (filaments) were placed on modified WPM. On these media calli developed, which were transferred to embryos on modified WPM with2.5µM BAP. Zygotic embryos of oak placed on modified WPM regenerated embryos on their surface. Some of the horse-chestnut embryos were potted in soil and are growing in the greenhouse.
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