Advertisement

Stump Sprouts: A Valuable Source for Clonal Propagation and Germplasm Preservation of Adult Sessile Oak

  • Karl Gebhardt
  • Birgit Heineker
  • Horst Weisgerber
Part of the NATO ASI Series book series (NSSA, volume 210)

Abstract

From a natural stand of sessile oak ten continuously proliferating shoot tip cultures were established from stump sprouts of originally 24 selected genotypes. The rate of multiplication per subculture period (4 weeks) varied between 1.08 to 1.70. After six subculture intervals a total number of 440 elongated shoots proliferated from 79 established shoot tips. Shoot tip cultures were stored aseptically at growth retarding conditions. From one genotype rooted plantlets were produced from elongated shoots by a one minute dip treatment using 500 ppm indolebutyric acid.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature

  1. 1.
    Chalupa, V., 1984, In vitro propagation of oak (Ouercus robur. L.) and linden (Tilia cordata Mill.). Biol. Plant. 26: 374–377.CrossRefGoogle Scholar
  2. 2.
    Civinova, B. and Sladky, Z., 1987, A study of the regeneration capacity of oak (Ouercus robur L.). Scripta FAC.SCI.NAT. Univ. PURK. BRUN (Biologia) 17: 103–110.Google Scholar
  3. 3.
    Gebhardt, K., 1989, Fortschritte und Perspektiven der Mikrovermehrung von Forstpflanzen. Die Holzzucht 43: 6–12.Google Scholar
  4. 4.
    Gresshoff, P.M. and Doy, C.H., 1972, Development and differentation of haploid Lycopersicon esculentum. Planta 107: 161–170.CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Meier-Dinkel, A., 1987, In-vitro-Kultur und Weiterkultur von Stieleiche (Ouercus robur L.) und Traubeneiche (Ouercus petraea (Matt.) Liebl.). Allg. Forst u. Jagdzeitung 158: 199–204.Google Scholar
  6. 6.
    Vieitez, A.M., San-Jose, M.C. and Vieitez, E., 1985, In vitro plantlet regeneration from juvenile and mature Ouercus robur L. J. Hort. Science 60: 99–106.Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Karl Gebhardt
    • 1
  • Birgit Heineker
    • 1
  • Horst Weisgerber
    • 1
  1. 1.Forschungsinstitut für schnellwachsende BaumartenHann. MündenGermany

Personalised recommendations