Protoplast Culture from Fagus, Ulmus and Abies

  • Hermann Lang
  • Hans Willy Kohlenbach
Part of the NATO ASI Series book series (NSSA, volume 210)


In times of progressing forest decline efforts on the preservation of genetic resources and on new approaches to the vegetative propagation from proven mature trees gain in significance. Since most forest trees are difficult to propagate from stem cuttings past their juvenile stage in-vitro-culture methods are currently being applied. Protoplast culture would allow the establishment of in-vitro-techniques for genetic preservation, vegetative propagation and somatic hybridization, if in addition methods for plant regeneration from protoplast-derived clones could be developed.


Embryogenic Callus Somatic Hybridization Vegetative Propagation Fagus Sylvatica Stem Cutting 


  1. 1.
    Gupta, P.K., Dandekar, A.M., and Durzan, D.J., 1988, Somatic embryo formation and transient expression of a luciferase gene in Douglas fir and loblolly pine protoplasts. Plant Science 58: 85–92.CrossRefGoogle Scholar
  2. 2.
    Kao, K.N., and Michayluk, M.R., 1975, Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110.CrossRefGoogle Scholar
  3. 3.
    Kohlenbach, H.W., and Lang, H., 1988, Protoplasten-Technik für Forstpflanzen? Allg. Forst Zeitschr. Heft 49, 1355–1357.Google Scholar
  4. 4.
    Lang, H., and Kohlenbach, H.W., 1988, Callus formation from mesophyll protoplasts of Fagus sylvatica L. Plant Cell Reports 7: 485–488.CrossRefGoogle Scholar
  5. 5.
    Lang, H., and Kohlenbach, H.W., 1989, Cell differentiation in protoplast cultures from embryogenic callus of Abies alba L. Plant Cell Reports 8: 120–123.CrossRefGoogle Scholar
  6. 6.
    Schuller, A., Reuther, G., and Geier, T., 1989, Somatic embryogenesis from seed expiants of Abies alba. Plant Cell Tiss. Org. Cult. 17: 53–58.Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Hermann Lang
    • 1
  • Hans Willy Kohlenbach
    • 1
  1. 1.Botanisches InstitutJ.W. Goethe-UniversitätFrankfurt am MainGermany

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