Influences of Subculturing Period and Different Culture Media on Cold Storage Maintenance of Populus Alba X P. grandidentata Plantlets
Continuous subculture is labor intensive and requires extensive culture space. Cold storage of in vitro cultured Populus plantlets could serve to alleviate the maintenance requirements of an established micropropagation system, as well as provide methods to facilitate germplasm conservation. In vitro cultured hybrid poplar, Populus alba X P. grandidentata could be stored at 4oC air temperature in darkness for 24 months and still recover suitable multiplication potential. Subculturing period preceding cold storage, plantlet condition, and culturing medium all had an important influence on survival at 4°C in darkness. A one-month subculturing period preceding cold storage was better than 0-month or 2-month subculturing period preceding cold storage. Shoot proliferation medium was better than rooting medium for long term cold storage. After 24 months storage, a 70% survival percentage was obtained with plantlets possessing 4–6 axillary branching shoots that were subcultured on shoot proliferation medium for one month preceding cold storage.
KeywordsCold Storage Hybrid Poplar Axillary Shoot Plant Cell Tissue Organ Culture Junk Publisher
Unable to display preview. Download preview PDF.
- 1.Aitken-Christie, J., and Gleed, J. A., 1984, Uses for micropropagations of juvenile radiata pine in New Zealand, Pages 47–57 in Proc. International Symp. Recent Advances in Forest Biotechnology, Traverse Ciy, MI.Google Scholar
- 4.Chun, Y. W., 1987, Biotechnology applications of Populus micropropagation, Ph.D. Dissertation, Iowa State Univ., Ames, Iowa 149 pp.Google Scholar
- 5.Chun, Y. W., and Hall, R. B., 1984, Survival and early growth of Populus alba X P. grandidentata in vitro cultured plantlets in soil, J. Korean For, Soc. 66:1–7.Google Scholar
- 6.Chun, Y. W., and Hall, R. B., 1986, Low temperature storage of in vitro cultured hybrid poplar, Populus alba X P. grandidentata plant-lets. Page 13 in D. A. Sommers, B. G. Gengenbach, D. D. Biesboer, W. P. Hackett, and G. E. Green, eds. Proc. Sixth International Congress of Plant Tissue and Cell Culture. Univ. of Minn., Minneapolis, MN.Google Scholar
- 8.Druart, P., 1985, In vitro germplasm preservation technique for fruit trees. Pages 149–154 in A. Schafer-Menuhr, ed. In vitro techniques: Propagation and long term storage. Martinus Nijohoff/Dr W. Junk Publishers, Boston.Google Scholar
- 10.Hall, R. B., Hilton, G. D., and Maynard, C. A., 1982, Construction lumber from hybrid aspen plantations in the Central States. J. of Forestry 80:291–294.Google Scholar
- 11.Lundergan, C., and Janick, J., 1979, Low temperature storage of in vitro apple shoots, Hort Science 14:514.Google Scholar
- 13.Mix, G., 1985, Preservation of old potato varieties. Pages 149–154 in A. Schafer-Menuhr, ed. In vitro techniques: Propagation and long term storage. Martinus Nijohoff/Dr W. Junk Publishers, Boston.Google Scholar
- 15.Preil, W., and Hoffmann, M., 1985, In vitro storage in chrysanthemum breeding and propagaion. Pages 161–166 in A. Schafer-Menuhr, ed. In vitro techniques: Propagation and long term storage. Martinus Nijohoff/Dr W. Junk Publishers, Boston.Google Scholar
- 16.Wilkins, C. P., and Dodds, J. H., 1983, The application of tissue culture techniques to plant genetic conservation. Sci. Prog, Oxf. 68:259–284.Google Scholar
- 17.Withers, L. A., 1985, Long-term storage of in vitro culture. Pages 137–148 in A. Schafer-Menuhr, ed. In vitro techniques: Propagation and long term storage. Martinus Nijohoff/Dr W. Junk Publishers, Boston.Google Scholar