Application of Biotechnology to Preservation of Forest Tree Germplasm

  • M. R. Ahuja
Part of the NATO ASI Series book series (NSSA, volume 210)


In view of large-scale forest decline, world-wide, it is necessary to preserve forest tree germplasm for the present and future. Preservation may be carried out in situ (on site; by natural regeneration or by propagation via seed or vegetative parts) or ex situ (away from the original site; in seed or clonal orchards, or by storage of seed, pollen, vegetative parts, or DNA). In this paper approaches to cryopreservation (storage in liquid nitrogen) of seeds and dormant buds of forest tree species are presented.


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  1. 1.
    Ahuja, M. R., 1986, Storage of forest tree germplasm in liquid nitrogen (-196 C). Silvae Genet. 35: 249–251.Google Scholar
  2. 2.
    Ahuja, M. R., 1986, Aspen. In: Handbook of Plant Cell Culture, D. A. Evans W. R. Sharp and P. J. Ammirato (Eds.), Macmillan Publishing Company, New York, pp. 626–651.Google Scholar
  3. 3.
    Ahuja, M. R., 1988, Differential growth response of aspen clones stored at sub-zero temperatures. In: Somatic Cell Genetics of Woody Plants, M. R. Ahuja (Ed.), Kluwer Academic Publishers, Dordrecht, pp. 173–180.CrossRefGoogle Scholar
  4. 4.
    Ahuja, M. R., 1989, Storage of forest tree germplasm at sub-zero temperatures. In: Application of Biotechnology in Forestry and Agriculture, V. Dhawan (Ed.), Plenum Press, New York, pp215–228.Google Scholar
  5. 5.
    Ahuja, M. R., 1990, Air pollution and mammal resistant genes. In: Genetics and Molecular Biology of Interactions between Harmful Agents and Trees, H. S. Manabb (Ed.), Springer Verlag, Berlin, New York (in press).Google Scholar
  6. 6.
    Ahuja, M. R. and Muhs, H. J., 1989, Biotechnologische Verfahren zur besseren Evaluierung, Erhaltung und Nutzung von forstliche Genressourcen. Berichte über Landwirtschaft, 201 Sonderheft. Verlag Paul Parey, Hamburg. 414–422.Google Scholar
  7. 7.
    Aitkin-Christie, J. and Singh, A. P., 1987, Cold storage of tissue cultures. In: Cell and Tissue Culture in Forestry, Volume 1, J. M. Bonga and D. J. Durzan (Eds.), Martinus Nijhoff Publishers, Dordrecht, pp. 285–304.CrossRefGoogle Scholar
  8. 8.
    Berichte des bundesministers für Ernährung, Landwirtschaft und Forsten, 1986, Waldzustandsbericht — Ergebnisse der Waldschadenserhebung. Landwirtschaftsverlag, Münster-Hiltrup 4Google Scholar
  9. 9.
    Berichte des Bundesministers für Ernährung, Landwirtschaft und Forsten, 1987, Waldzustandsbericht — Ergebnisse der Waldschadenserhebung. Landwirtschaftsverlag, Münster-Hiltrup.Google Scholar
  10. 10.
    Krugman, S. L., 1985, Policies, strategies, and means of genetic conservation in forestry. In: Plant Genetic Resources, C. W. Yeatman, D. Kafton and G. Wilkes (Eds.), AAAAS Symposium, Westview Press, Boulder, Colorado, pp. 71–78.Google Scholar
  11. 11.
    Ledig, F. T., 1988, The conservation of diversity in forest trees. Bioscience 38: 471–479.CrossRefGoogle Scholar
  12. 12.
    Melchior, G. H., Muhs, H. J. and Stephan, B. R., 1986, Tactics for the conservation of forest gene resources in the Federal Republic of Germany. For. Eco. Manag. 17: 73–81.Google Scholar
  13. 13.
    Millar, C. I., 1991, Conservation of germplasm in forest trees. In: Clonal Forestry: Genetics, Biotechnology and Application, M. R. Ahuja and W. J. Libby (Eds.), Springer Verlag (in press).Google Scholar
  14. 14.
    Preil, W. and Hoffmann, M., 1985, In vitro storage in Chrysanthemum breeding and propagation. In: In Vitro Techniques — Propagation and Long Term Storage, A. Schafer-Menuhr (Ed.), Martinus Nijhoff Publishers, Dordrecht, pp. 161–165.Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • M. R. Ahuja
    • 1
  1. 1.Federal Research Centre for Forestry and Forest ProductsInstitute of Forest Genetics and Forest Tree breedingGrosshansdorfGermany

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