Approaches to Studying DNA Elements Associated with Light-Regulated Gene Expression in Conifers
In many angiosperms light regulates the expression of genes coding for the small subunit of ribulose bis-phosphate carboxylase (rbcS) and chlorophyll a/b-binding protein (cab). Upstream DNA sequences of the rbcS and cab genes, associated with transcriptional regulation, are reviewed and the possible role that these sequences have in conifer gene regulation are discussed. A DNA sequence associated with light-regulated gene expression in angiosperms, called box-II, was found in the upstream region of a larch rbcS gene. Using a double reporter system, we present a method that can assign a functional role to the box-II, as well as to other sequences in the upstream region of the larch rbcS gene. This double reporter system has potential in examining the upstream regions of both rbcS and cab genes for sequences that are associated with transcriptional regulation in conifers.
KeywordsUpstream Region Bisphosphate Carboxylase rbcS Gene Upstream Portion Larix Laricina
Unable to display preview. Download preview PDF.
- 6.Gallager, T.F. and Ellis, R.J., 1982, Light-Stimulated Transcription of Genes for Two Chloroplast Polypeptides in Isolated Pea Leaf Nuclei. EMBO J. 1:1493–1498.Google Scholar
- 9.Green, P.J., Yong, M-H., Cuozzo, M., Kano-Murakami, Y., Silverstein, P., and Chua, N-H., 1988, Binding Site Requirements for Pea Nuclear Protein Factor GT-1 Correlate with Sequences Required for Light-Dependent Transcriptional Activation of the RbcS-3A Gene. EMBO J. 13:4035–4044.Google Scholar
- 11.Hutchison, K.W. (personal communication).Google Scholar
- 13.Hutchison, K.W., Harvie, P., Singer, P.B. and Greenwood, M.S., 1989, Structure and Expression of Light-regulated Genes During Growth and Maturation of Larix laricina. Abstract. IUFRO Workshop on Molecular Biology of Forest Trees. Riksgransen, Lappland, Sweden. June 11 to 14, 1989.Google Scholar
- 16.Kirby, E.G., Campbell, M.A., and Penchel, R.K., 1989, The Isolation and Culture of Protoplasts of Forest Tree Species. In: Biotechnology in Agriculture and Forestry: vol. 8. Plant Protoplasts and Engineering I. (Y.P.S. Baja ed.). Springer Verlag, Berlin pp. 262–274.Google Scholar
- 27.Wilson, S.M., Thorpe, T.A. and Moloney, M.M., 1989, PEG-Mediated Expression of GUS and CAT Genes in Protplasts from Embryogenic Suspension Cultures of Picea glauca. Plant Cell Rep. 7:704–707.Google Scholar
- 28.Yamamoto, N., Kano-Murakami, Y., Matsuoka, M., Ohashi, Y., and Tanaka, Y., 1988, Nucleotide Sequence of a Full Length cDNA Clone of Ribulose Bisphosphate Carboxylase Small Subunit Gene form Green Dark-Grown Pine (Pinus tunbergii) Seedling (Abstract). Nucleic Acids Res. 16:11830.PubMedCrossRefGoogle Scholar
- 29.Yamamoto, N., Matsuoka, M., Kano-Murakami, Y., Tanaka, Y. and Ohashi, Y., 1988, Nucleotide Sequence of a Full Length cDNA Clone of Light Harveting Chlorophyll a/b Binding Protein Gene From Green Dark-Grown Pine (Pinus tunbergii) Seedling (Abstract). Nucleic Acid Res. 16:11829.PubMedCrossRefGoogle Scholar