Approaches to Studying DNA Elements Associated with Light-Regulated Gene Expression in Conifers

  • M. A. Campbell
  • D. B. Neale
Part of the NATO ASI Series book series (NSSA, volume 210)


In many angiosperms light regulates the expression of genes coding for the small subunit of ribulose bis-phosphate carboxylase (rbcS) and chlorophyll a/b-binding protein (cab). Upstream DNA sequences of the rbcS and cab genes, associated with transcriptional regulation, are reviewed and the possible role that these sequences have in conifer gene regulation are discussed. A DNA sequence associated with light-regulated gene expression in angiosperms, called box-II, was found in the upstream region of a larch rbcS gene. Using a double reporter system, we present a method that can assign a functional role to the box-II, as well as to other sequences in the upstream region of the larch rbcS gene. This double reporter system has potential in examining the upstream regions of both rbcS and cab genes for sequences that are associated with transcriptional regulation in conifers.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Alosi, M.C., Neale, D.B. and Kinlaw, C.S., 1990, Steady State Expression of cab Genes in Douglas-fir is Not Strongly Regulated by Light. Plant Physiol 93:829–832.CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Castresana, C., Garcia-Luque, I., Alonso, E., Malik, V.S. and Cashmore, A.R., 1988, Both Positive and Negative Regulatory Elements Mediate Expression of a Photoregulated CAB Gene From Nicotiana plumbaginifolia. EMBO J. 7:1929–1936.PubMedPubMedCentralGoogle Scholar
  3. 3.
    Dandekar, A.M., Gupta, P.M., Durzan, D.J. and Knauf, V., 1987, Transformation and Foreign Gene Expression in Micropropagated Douglas-fir (Pseudotsuga menziesii). Bio/Tech. 5:587–590.Google Scholar
  4. 4.
    Dean, C., Pichersky, E. and Dunsmuir, P., 1989, Structure Evolution, and Regulation of RbcS Genes in Higher Plants. Ann. Rev. Plant Physiol. 40:415–439.CrossRefGoogle Scholar
  5. 5.
    Fluhr, R., Kuhlemeier, C., Nagy, F. and Chua, N-H., 1986, Organ-Specific and Light-Induced Expression of Plant Genes. Science 232:1106–1112.CrossRefPubMedGoogle Scholar
  6. 6.
    Gallager, T.F. and Ellis, R.J., 1982, Light-Stimulated Transcription of Genes for Two Chloroplast Polypeptides in Isolated Pea Leaf Nuclei. EMBO J. 1:1493–1498.Google Scholar
  7. 7.
    Gilmartin, P.M., Sarokin, L., Memelink, J., and Chua, N-H., 1990, Molecular Light Switches for Plant Genes. Plant Cell 2:369–378.CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Giuliano, G., Pichersky, E., Malik, V.S., Timko, M.P., Scolnik, P.A. and Cashmore, A.R., 1988, An Evolutionarily Conserved Protein Binding Sequence Upstream of a Plant Light-Regulated Gene. Proc. Natl. Acad. Sci.(USA) 85:7089–7093.CrossRefPubMedCentralGoogle Scholar
  9. 9.
    Green, P.J., Yong, M-H., Cuozzo, M., Kano-Murakami, Y., Silverstein, P., and Chua, N-H., 1988, Binding Site Requirements for Pea Nuclear Protein Factor GT-1 Correlate with Sequences Required for Light-Dependent Transcriptional Activation of the RbcS-3A Gene. EMBO J. 13:4035–4044.Google Scholar
  10. 10.
    Gupta, P.K., Dandekar, A.M. and Durzan, D.J., 1988, Somatic Proembryo Formation and Transient Expression of a Luciferase Gene in Douglas-fir and Loblolly Pine Protoplasts. Plant Science 58:85–92.CrossRefGoogle Scholar
  11. 11.
    Hutchison, K.W. (personal communication).Google Scholar
  12. 12.
    Hutchison, K.W., Harvie, P.D., Singer, P.B., Brunner, A.F., and Greeenwood, M.S., 1990, Nucleotide Sequence of the Small Subunit of Ribulose-l,5-bisphosphate Carboxylase from the Conifer Larix laricina. Plant Molec. Biol. 14:281–284.CrossRefGoogle Scholar
  13. 13.
    Hutchison, K.W., Harvie, P., Singer, P.B. and Greenwood, M.S., 1989, Structure and Expression of Light-regulated Genes During Growth and Maturation of Larix laricina. Abstract. IUFRO Workshop on Molecular Biology of Forest Trees. Riksgransen, Lappland, Sweden. June 11 to 14, 1989.Google Scholar
  14. 14.
    Jansson, S. and Gustafsson, P., 1990, Type I and Type II Genes for the Chlorophyll a/b-binding Protein in the Gymnosperm Pinus sylvestris (Scots pine): cDNA Cloning and Sequence Analysis. Plant Mol. Biol. 14:287–296.CrossRefPubMedGoogle Scholar
  15. 15.
    Jefferson, R.A., 1987, Assaying Chimeric Genes in Plants: The Gus Gene Fusion System. Plant Molec. Biol. Rep. 5:387–405.CrossRefGoogle Scholar
  16. 16.
    Kirby, E.G., Campbell, M.A., and Penchel, R.K., 1989, The Isolation and Culture of Protoplasts of Forest Tree Species. In: Biotechnology in Agriculture and Forestry: vol. 8. Plant Protoplasts and Engineering I. (Y.P.S. Baja ed.). Springer Verlag, Berlin pp. 262–274.Google Scholar
  17. 17.
    Klein, T.M., Gradziel, T., Fromm, M.E. and Sanford, J.C., 1988, Factors Influencing Gene Delivery into Zea mays Cells by High-Velocity Microprojectiles. Bio/Tech. 6:559–563.Google Scholar
  18. 18.
    Kuhlemeier, C., Green, P.J., and Chua, N-H., 1987, Regulation of Gene Expression in Higher Plants. Ann Rev. Plant Physiol. 38:221–257.CrossRefGoogle Scholar
  19. 19.
    Manzara, T. and Gruissem, W., 1988, Organization and Expression of the Genes Encoding Ribulose-1,5-Bisphosphate Carboxylase in Higher Plants. Photosyn. Res. 16:117–139.CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Nagy, F., Fluhr, R., Kuhlemeier, C., Kay, S., Boutry, M., Poulsen, C. and Chua, N-H., 1986, Cis-acting Elements for Selective Expression of Two Photosynthetic Genes in Transgenic Plants. Phil. Trans. R. Soc. Lond. 314:493–500.CrossRefGoogle Scholar
  21. 21.
    Ow, D.W., Jacobs, J.D. and Howell, S.H., 1987, Functional Regions of the Cauliflower Mosaic Virus 35S RNA Promoter Determined by Use of the Firefly Luciferase Gene as a Reporter of Promoter Activity. Proc. Natl. Acad. Sci.(USA) 84:4870–4874.CrossRefGoogle Scholar
  22. 22.
    Sederoff, R., Stomp, A.M., Chiton, W.C. and Moore, L.W., 1986, Gene Transfer into Loblolly Pine by Agrobacterium tumefaciens. Bio/Tech. 4:647–649.Google Scholar
  23. 23.
    Simpson, J. Van Montagu, M., Herrera-Estrella, L., 1986, Photosynthesis-Asociated Gene Families: Differences in Response to Tissue-Specific and Environmental Factors. Science 233:34–38.CrossRefPubMedGoogle Scholar
  24. 24.
    Thompson, W.F., Everett, M., Polans, N.O., Jorgensen, R.A., Palmer, J.D., 1983, Phytochrome Control of RNA Levels in Developing Pea and Mung-bean Leaves. Planta 158:487–500.CrossRefPubMedGoogle Scholar
  25. 25.
    Tobin, E.M. and Silverthorne, J., 1985. Light Regulation of Gene Expression in Higher Plants. Annu. Rev. Plant Physiol. 36:569–593.CrossRefGoogle Scholar
  26. 26.
    Weising, K., Schell, J. and Kahl, G., 1988, Foreign Genes in Plants: Transfer, Structure, Expression and Applications. Annu. Rev. Genet. 22:421–477.CrossRefPubMedGoogle Scholar
  27. 27.
    Wilson, S.M., Thorpe, T.A. and Moloney, M.M., 1989, PEG-Mediated Expression of GUS and CAT Genes in Protplasts from Embryogenic Suspension Cultures of Picea glauca. Plant Cell Rep. 7:704–707.PubMedGoogle Scholar
  28. 28.
    Yamamoto, N., Kano-Murakami, Y., Matsuoka, M., Ohashi, Y., and Tanaka, Y., 1988, Nucleotide Sequence of a Full Length cDNA Clone of Ribulose Bisphosphate Carboxylase Small Subunit Gene form Green Dark-Grown Pine (Pinus tunbergii) Seedling (Abstract). Nucleic Acids Res. 16:11830.CrossRefPubMedPubMedCentralGoogle Scholar
  29. 29.
    Yamamoto, N., Matsuoka, M., Kano-Murakami, Y., Tanaka, Y. and Ohashi, Y., 1988, Nucleotide Sequence of a Full Length cDNA Clone of Light Harveting Chlorophyll a/b Binding Protein Gene From Green Dark-Grown Pine (Pinus tunbergii) Seedling (Abstract). Nucleic Acid Res. 16:11829.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • M. A. Campbell
    • 1
  • D. B. Neale
    • 1
  1. 1.Pacific Southwest Research Station, Institute of Forest GeneticsU.S.D.A. Forest ServiceBerkeleyUSA

Personalised recommendations