Insertion of the Maize Transposable Element AC Into Poplar

  • Glenn T. Howe
  • Steven H. Strauss
  • Barry Goldfarb
Part of the NATO ASI Series book series (NSSA, volume 210)


The maize transposable element Ac (Activator) was inserted into hybrid poplar (Populus alba x P. grandidentata) using cocultivation of suspension cultures with Agrobacterium tumefaciens. Southern blot analysis indicates that six of eight hygromycin-resistant callus lines contain stably integrated foreign DNA. The number of inserted T-DNA copies ranges from approximately 1 to 10. Southern blot analysis suggests that Ac has excised from its original location in the T-DNA. We are currently studying transposition of Ac in transgenic shoots. We believe this to be the first report of transposon activity in a woody plant.


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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Glenn T. Howe
    • 1
  • Steven H. Strauss
    • 1
  • Barry Goldfarb
    • 1
  1. 1.Department of Forest ScienceOregon State UniversityCorvallisUSA

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