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Insertion of the Maize Transposable Element AC Into Poplar

  • Glenn T. Howe
  • Steven H. Strauss
  • Barry Goldfarb
Part of the NATO ASI Series book series (NSSA, volume 210)

Abstract

The maize transposable element Ac (Activator) was inserted into hybrid poplar (Populus alba x P. grandidentata) using cocultivation of suspension cultures with Agrobacterium tumefaciens. Southern blot analysis indicates that six of eight hygromycin-resistant callus lines contain stably integrated foreign DNA. The number of inserted T-DNA copies ranges from approximately 1 to 10. Southern blot analysis suggests that Ac has excised from its original location in the T-DNA. We are currently studying transposition of Ac in transgenic shoots. We believe this to be the first report of transposon activity in a woody plant.

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References

  1. 1.
    An, G., 1985, High efficiency transformation of cultured tobacco cells. Plant Physiol. 79: 568–570.CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    An, G., Ebert, P.R., Mitra, A., and Ha, S.B., 1988, Binary vectors. In: “Plant Molecular Biology Manual,” S.B. Gelvin and R.A. Schilperoort, eds., Kluwer Acad. Pub., Dordrecht, pp. A3/1-A3/19.Google Scholar
  3. 3.
    Baker, B., Schell, J., Lorz, H., and Fedoroff, N., 1986, Transposition of the maize controlling element Activator in tobacco. Proc. Natl. Acad. Sci. USA 83: 4844–4848.CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Baker, B., Coupland, G., Fedoroff, N., Starlinger, P., and Schell, J., 1987, Phenotypic assay for excision of the maize controlling element Ac in tobacco. EMBO J. 6: 1547–1554.PubMedPubMedCentralGoogle Scholar
  5. 5.
    Belzile, F., Lassner, M.W., Tong, Y., Khush, R., and Yoder, J.I., 1989, Sexual transmission of transposed Activator elements in transgenic tomatoes. Genetics 123: 181–189.PubMedPubMedCentralGoogle Scholar
  6. 6.
    Bonas, U., Sommer, H., and Saedler, H., 1984, The 17-kb Taml element of Antirrhinum majus induces a 3-bp duplication upon integration into the chalcone synthase gene. EMBO J. 3: 1015–1019.PubMedPubMedCentralGoogle Scholar
  7. 7.
    Coupland, G., Baker, B., Schell, J., and Starlinger, P., 1988, Characterization of the maize transposable element Ac by internal deletions. EMBO J. 7: 3653–3659.PubMedPubMedCentralGoogle Scholar
  8. 8.
    Coupland, G., Plum, C., Chatterjee, S., Post, A., and Starlinger, P., 1989, Sequences near the termini are required for transposition of the maize transposon Ac in transgenic tobacco plants. Proc. Natl. Acad. Sci. USA 86: 9385–9388.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Dekeyser, R., Claes, B., Marichal, M., Van Montagu, M., and Caplan, A., 1989, Evaluation of selectable markers for rice transformation. Plant Physiol. 90: 217–223.CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Fedoroff, N., 1983, Controlling elements in maize. In: “Mobile Genetic Elements,” J.A. Shapiro, ed., Academic Press, New York, pp. 1–63.Google Scholar
  11. 11.
    Fillatti, J.J., Sellmer, J., McCown, B., Haissig, B., and Cornai, L., 1987, Agrobacterium mediated transformation and regeneration of Populus. Mol. Gen. Genet. 206: 192–199.CrossRefGoogle Scholar
  12. 12.
    Finnegan, E.J., Taylor, B.H., Dennis, E.S., and Peacock, W.J., 1988, Transcription of the maize transposable element Ac in maize seedlings and in transgenic tobacco. Mol. Gen. Genet. 212: 505–509.CrossRefGoogle Scholar
  13. 13.
    Finnegan, E.J., Taylor, B.H., Craig, S., and Dennis, E.S., 1989, Transposable elements can be used to study cell lineages in transgenic plants. Plant Cell 1: 757–764.CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Frey, M., Tavantzis, S.M., and Saedler, H., 1989, The maize En-1/Spm element transposes in potato. Mol. Gen. Genet., 217: 172–177.CrossRefPubMedGoogle Scholar
  15. 15.
    Gritz, L., and Davies, J., 1983, Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25: 179–188.CrossRefPubMedGoogle Scholar
  16. 16.
    Haring, M.A., Gao, J., Volbeda, T., Rommens, C.M.T., Nijkamp, H.J.J., and Hille, J., 1989, A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants. Plant Mol. Biol. 13: 189–201.CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Hauptmann, R.M., Vasil, V., Ozias-Akins, P., Tabaeizadeh, Z., Rogers, S., Fraley, R.T., Horsch, R.B., and Vasil, I.K., 1988, Evaluation of selectable markers for obtaining stable transformants in the Gramineae. Plant Physiol. 86: 602–606.CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Hehl, R. and Baker, B., 1989, Induced transposition of Ds by a stable Ac in crosses of transgenic tobacco plants. Mol. Gen. Genet. 217: 53–59.CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Jones, J.D.G., Carland, F.M., Maliga, P. and Dooner, H.K., 1989, Visual detection of transposition of the maize element Activator (Ac) in tobacco seedlings. Science 244: 204–207.CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Joos, H., Timmermann, B., Van Montagu, M., and Schell, J., 1983, Genetic analysis of transfer and stabilization of Agrobacterium DNA in plant cells. EMBO J. 2: 2151–2160.PubMedPubMedCentralGoogle Scholar
  21. 21.
    Knapp, S., Coupland, G., Uhrig, H., Starlinger, P., and Salamini, F., 1988, Transposition of the maize transposable element Ac in Solanum tuberosum. Mol. Gen. Genet. 213: 285–290.CrossRefGoogle Scholar
  22. 22.
    Labarca, C., and Paigen, K., 1980, A simple, rapid, and sensitive DNA assay procedure. Anal. Biochem. 102: 344–352.CrossRefPubMedGoogle Scholar
  23. 23.
    Lassner, M.W., Palys, J.M., and Yoder, J.I., 1989, Genetic transactivation of Dissociation elements in transgenic tomato plants. Mol. Gen. Genet. 218: 25–32.CrossRefGoogle Scholar
  24. 24.
    Lloyd, G.B., and McCown, B.H., 1980, Commercially-feasible micro-propagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc. Intl. Plant Prop. Soc. 30: 421–427.Google Scholar
  25. 25.
    Maniatis, T., Fritsch, E.F., and Sambrook, J., 1982, eds., “Molecular Cloning, a Laboratory Manual.” Cold Spring Harbor, New York.Google Scholar
  26. 26.
    Martin, C, Carpenter, R., Sommer, H., Saedler, H., and Coen, E.S., 1985, Molecular analysis of instability in flower pigmentation of Antirrinum majus, following isolation of the pallida locus by transposon tagging. EMBO J. 4: 1625–1630.PubMedPubMedCentralGoogle Scholar
  27. 27.
    Masson, P., and Fedoroff, N., 1989, Mobility of the maize Suppressor-mutator element in transgenic tobacco cells. Proc. Natl. Acad. Sci. USA 86: 2219–2223.CrossRefPubMedPubMedCentralGoogle Scholar
  28. 28.
    Masson, P., Rutherford, G., Banks, J.A., and Fedoroff, N., 1989, Essential large transcripts of the maize Spm transposable element are generated by alternative splicing. Cell 58: 755–765.CrossRefPubMedGoogle Scholar
  29. 29.
    Murashige, T., and Skoog, F., 1962, A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–492.CrossRefGoogle Scholar
  30. 30.
    Murray, M.G., and Thompson, W.F., 1980, Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321–4325.CrossRefPubMedPubMedCentralGoogle Scholar
  31. 31.
    Parsons, T.J., Sinkar, V.P., Stettier, R.F., Nester, E.W., and Gordon, M.P., 1986, Transformation of poplar by Agrobacterium tumefaciens. Bio/Technology 4: 533–536.Google Scholar
  32. 32.
    Pereira, A., and Saedler, H., 1989, Transpositional behavior of the maize En/Spm element in transgenic tobacco. EMBO J. 8: 1315–1321.PubMedPubMedCentralGoogle Scholar
  33. 33.
    Pollock, K., Barfield, D.G., Robinson, S.J., and Shields, R., 1985, Transformation of protoplast-derived cell colonies and suspension cultures by Agrobacterium tumefaciens. Plant Cell Rep. 4: 202–205.CrossRefPubMedGoogle Scholar
  34. 34.
    Reed, K.C., and Mann, D.A., 1985, Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 13: 7207–7221.CrossRefPubMedPubMedCentralGoogle Scholar
  35. 35.
    Reynaerts, A., De Block, M., Hernalsteens, J.-P., and Van Montagu, M., 1988, Selectable and screenable markers. In: “Plant Molecular Biology Manual,” S.B. Gelvin and R.A. Schilperoort, eds., Kluwer Acad. Pub., Dordrecht, pp. A9/1-A9/16.Google Scholar
  36. 36.
    Russell, J.A., and McCown, B.H., 1986, Techniques for enhanced release of leaf protoplasts in Populus. Plant Cell Rep. 5: 284–287.CrossRefPubMedGoogle Scholar
  37. 37.
    Scott, R.J., and Draper, J., 1987, Transformation of carrot tissues derived from proembryogenic suspension cells: A useful model system for gene expression studies in plants. Plant Mol. Biol. 8: 265–274.CrossRefGoogle Scholar
  38. 38.
    Sellmer, J.C., and McCown, B.H., 1989, Transformation in Populus spp. In: “Biotechnology in Agriculture and Forestry vol. 9: Plant Protoplasts and Genetic Engineering II”, Y.P.S. Bajaj, ed., Springer Verlag, Berlin, pp. 155–172.Google Scholar
  39. 39.
    van den Elzen, P.J.M., Townsend, J., Lee, K.Y., and Bedbrook, J.R., 1985, A chimaeric hygromycin resistance gene as a selectable marker in plant cells. Plant Mol. Biol. 5: 299–302.CrossRefPubMedPubMedCentralGoogle Scholar
  40. 40.
    Van Sluys, M.A., and Tempe, J., 1989, Behavior of the maize transposable element Activator in Daucus carota. Mol. Gen. Genet. 219: 313–319.Google Scholar
  41. 41.
    Van Sluys, M.A., Tempe, J., and Fedoroff, N., 1987, Studies on the introduction and mobility of the maize Activator element in Arabidopsis thaliana and Daucus carota. EMBO J. 6: 3881–3889.PubMedPubMedCentralGoogle Scholar
  42. 42.
    Veiten, J., Veiten, L., Hain, R., and Schell, J., 1984, Isolation of a dual plant promoter fragment from the Ti plasmid of Agrobacterium tumefaciens. EMBO J. 3: 2723–2730.Google Scholar
  43. 43.
    Wienand, U., and Saedler, H., 1988, Plant transposable elements: unique structures for gene tagging and gene cloning. In: “Plant DNA Infectious Agents”, T. Hohn and J. Schell, eds., Springer, New York, pp. 205–228.Google Scholar
  44. 44.
    Yoder, J.I., Palys, J., Alpert, K., and Lassner, M., 1988, Ac transposition in transgenic tomato plants. Mol. Gen. Genet. 213: 291–296.CrossRefGoogle Scholar
  45. 45.
    Zambryski, P., Joos, H., Genetello, C., Leemans, J., Van Montagu, M., and Schell, J., 1983, Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO J. 2: 2143–2150.PubMedPubMedCentralGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Glenn T. Howe
    • 1
  • Steven H. Strauss
    • 1
  • Barry Goldfarb
    • 1
  1. 1.Department of Forest ScienceOregon State UniversityCorvallisUSA

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