Transfer of Foreign Genes into Yellow-Poplar (Liriodendron Tulipifera)
Yellow-poplar can be regenerated from tissue culture by somatic embryogenesis. The transformation of embryogenic cultures should allow the production of transgenic yellow-poplar plants. Direct gene transfer methods have been used to introduce the gene encoding ß-glucuronidase (GUS) into protoplasts (polyethyene-glycol mediated uptake, electroporation) and intact cells (microprojectile bombardment) from suspension cultures. In addition to other parameters, the physiological age of the protoplasts was found to be an important factor in the transient expression of GUS. Intact cells, bombarded with DNA-coated tungsten particles, were found to express the marker gene individually and in cell clusters.
KeywordsSomatic Embryogenesis Embryogenic Culture Decay Time Constant Microprojectile Bombardment Embryogenic Suspension Culture
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