Genetic Transformation of Trees via Direct Gene Transfer
The recent successes in genetically-engineering trees have in large part been due to advances in both the microculture and the gene transfer technologies. Besides regeneration, a critical problem with microculture has been developing efficient techniques for selecting cells and tissues that are expressing the inserted foreign genes. One solution has been the use of liquid overlays of the selective agent. In regards to gene transfer technologies, both vector-mediated and direct gene transfer techniques have been successful, particularly with Populus. However, the versatility and efficiency of particle bombardment appears to be a major advantage that may lead to the rapid adoption of such methods in biotechnology programs utilizing tree species.
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