Advertisement

Somatic Embryogenesis and Organogenesis of Embryonic Explants of Abies Alba and Acer Pseudoplatanus

  • Anton Grahsl
  • Josef Schmidt
  • Eva Wilhelm
Part of the NATO ASI Series book series (NSSA, volume 210)

Abstract

With Abies alba somatic embryos were induced from the hypocotyl of excised veil developed zygotic embryos. Extended growth of embryogenic cells was observed on auxin-free medium with 2.2 μM/1 BAP ± 2.3 μM/1 kinetin. Transplants from several of these cultures developed continuously numerous embryos with green cotyledons, but root development was generally disturbed. Direct organogenesis was observed on the same media. Wreaths of buds formed around the hypocotyls right beneath the base of the cotyledons. Little bud formation occurred on medium containing auxin.

Acer pseudoplatanus does not express the same vegetative regeneration capacity in vitro as in vivo. Nevertheless buds formed from the hypocotyl part of segmented zygotic embryos when grown on induction medium supplemented with 0.01 or 0.05 μM thidiazuron. Nearly 40% of cultures produced shoots up to 20 mm in length. Bud production and shoot growth continued on the original expiants or larger pieces of organogenetic callus, when transferred on the respective medium at monthly intervals.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature

  1. 1.
    Bornman, C.H., and Jansson, E., 1981, Regeneration of plants from the conifer leaf, Colloque sur la culture in vitro des essences forestieres, AFOCEL, Nangis, France, p. 41–55.Google Scholar
  2. 2.
    Gebhardt, K., Weisgerber, H., and Fröhlich, H. I., 1988, In vitro germination and production of embryogenic callus from liquid suspension cultures of Abies alba — silver fir, Poster Abstract, 4th Int. Conifer Tissue Culture Work Group, Saskatoon.Google Scholar
  3. 3.
    Gresshof, P.M., and Doy, C.H., 1972, Development and differentiation of haploid Lycopersicon esculentum (tomato), Planta, 17:161–170.CrossRefGoogle Scholar
  4. 4.
    Kerns, H. R., and Meyer, M. M. Jr., 1986, Tissue culture propagation of Acer x freemanii using thidiazuron to stimulate shoot tip proliferation, HortScience, 21:1209–1210.Google Scholar
  5. 5.
    Schuller A., Reuther, G., and Geier, Th., 1989, Somatic embryogenesis from seed expiants of Abies alba, Plant Cell, Tissue and Organ Culture, 17:53–58.Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Anton Grahsl
    • 1
  • Josef Schmidt
    • 1
  • Eva Wilhelm
    • 1
  1. 1.Austrian Research Centre Seibersdorf Ltd.SeibersdorfAustria

Personalised recommendations