Advertisement

Genetic and Morphogenic Stability of Plantlets Regenerated from Somatic Embryos of Norway Spruce (Picea abies)

  • L. H. Mo
  • S. von Arnold
Part of the NATO ASI Series book series (NSSA, volume 210)

Abstract

Embryogenic cultures were initiated from mature zygotic embryos of Picea abies. The somatic embryos in the embryogenic cultures were first stimulated to mature and then either to develop further into plantlets or to differentiate new embryogenic cultures. The procedure was repeated three times during two years. The ability to give rise to new embryogenic cultures or to develop into plantlets was similar for all somatic embryos irrespective of how long they had been cultured in vitro. The nuclear DNA content, measured in a flow cytometer, was estimated to 32 pg / Gl nuclei in seedlings developed from zygotic embryos. Nuclei isolated from embryogenic cultures and from plantlets regenerated from somatic embryos had the same DNA content as those isolated from seedlings.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Arnold von S., 1982, Factors influencing formation, development and rooting of adventitious shoots from embryos of Picea abies (L.) karst. Plant Sci. Lett. 27:275–287.CrossRefGoogle Scholar
  2. 2.
    Bayliss M.W., 1980, Chromosomal variation in plant tissues in culture. Int. Rev. Cytol. 11A:113–144.Google Scholar
  3. 3.
    Hakman L., von Arnold S. and Bengtsson A., 1984, Cytofluorometric measurements of nuclear DNA in adventitious buds and shoots of Picea abies regenerated in vitro. Physiol. Plant. 60:321–325.CrossRefGoogle Scholar
  4. 4.
    Lelu M.A., 1987, Variations morphologiques et génétiques chez Picea abies obtenues après embryogenèse somatique. In: Annales de Recherches Sylvicoles, AFOCEL, 35–47.Google Scholar
  5. 5.
    Mo L.H., von Arnold S. and Lagercrantz U., 1989, Morphogenic and genetic stability in longterm embryogenic cultures and somatic embryos of Norway spruce (Picea abies L. Karst). Plant Cell Reports 8:375–378.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Patel K.R. and Berlyn G.P., 1982, Genetic instability of multiple buds ofPinus coulteri regenerated from tissue culture. Can. J. For.Res. 12:93–101.CrossRefGoogle Scholar
  7. 7.
    Renfroe M.H. and Berlyn G.P., 1984, Stability of nuclear DNA content during adventitious shoot formation in Pinus taeda L. Am. J. Bot. 71 (2):268–272.CrossRefGoogle Scholar
  8. 8.
    Schuller A., Reuther G. and Geier T., 1989, Somatic embryogenesis from seed expiants of Abies alba. Plant Cell,Tissue and Organ Culture 17:53–58.Google Scholar
  9. 9.
    Ulrich I., Fritz B. and Ulrich W., 1988, Application of DNA fluorochromes for flow cytometric DNA analysis of plant protoplasts. Plant Science 55:151–158.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • L. H. Mo
    • 1
  • S. von Arnold
    • 1
  1. 1.Department of Forest GeneticsSwedish University of Agricultural SciencesUppsalaSweden

Personalised recommendations