Genetic and Morphogenic Stability of Plantlets Regenerated from Somatic Embryos of Norway Spruce (Picea abies)
Embryogenic cultures were initiated from mature zygotic embryos of Picea abies. The somatic embryos in the embryogenic cultures were first stimulated to mature and then either to develop further into plantlets or to differentiate new embryogenic cultures. The procedure was repeated three times during two years. The ability to give rise to new embryogenic cultures or to develop into plantlets was similar for all somatic embryos irrespective of how long they had been cultured in vitro. The nuclear DNA content, measured in a flow cytometer, was estimated to 32 pg / Gl nuclei in seedlings developed from zygotic embryos. Nuclei isolated from embryogenic cultures and from plantlets regenerated from somatic embryos had the same DNA content as those isolated from seedlings.
KeywordsSomatic Embryo Zygotic Embryo Adventitious Shoot Picea Abies Embryogenic Culture
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