Somatic Embryogenesis in Tissue Cultures of Euterpe Edulis Mart. (Palmae)

  • Miguel P. Guerra
  • Walter Handro
Part of the NATO ASI Series book series (NSSA, volume 210)


Somatic embryogenesis and further development of embryos to plantlets was studied using: young and mature embryos excised from seeds and inflorescences in 10 different stages until emergence. The influence of several factors such as mineral salts, nitrogen source, growth regulators, sucrose etc. were studied. The morphogenetical events were also analysed in their histological aspects. The main results can be summarized as follows: a) the embryogenic process has characteristic steps: firstly, the development of an embryogenic tissue, producing globular embryos, isolated or forming clusters. Depending on the hormonal balance, either a secondary embryogenic process can occur, or the development of the embryos to further stages until plantlets; b) very young inflorescences and also those ones near the emergence were unable to develop embryos; only certain intermediate stages can do this; c) immature embryos showed higher embryogenetic potential than mature ones. Requirements to control this process were: a) activated charcoal during the first two weeks of culture, and dark in the case of inflorescences; b) 2,4-D (50 mg.l-1) is the best condition to induce embryogenes is; c) further development of embryos, as well secondary embryogenesis occurred after lowering 2,4-D to 10 mg.l-1 and adding 2-iP (5 mg.l-1); d) further transfer to medium lacking growth regulators leads to the development of the embryos into plantlets.


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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Miguel P. Guerra
    • 1
  • Walter Handro
    • 1
  1. 1.Department of Botany, Institute of BiosciencesUniversity of São PauloSão PauloBrazil

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