Effect of Explant and Media on Initiation, Maintenance, and Maturation of Somatic Embryos in Pseudotsuga Menziesii (Mirb.) Franco (Douglas-fir)
embryogenic calli were initiated from immature zygotic embryos of Pseudotsuga menziesii (Mirb.) Franco (Douglas-fir). Only precotyledonary embryos gave rise to embryogenic cell lines that could be maintained and manipulated to yield cotyledonary somatic embryos. Neither intact ovules nor cotyledonary expiants were responsive to the four test media. embryogenic cell lines were initiated on modified MS medium supplemented with L-glutamine (500 mg/1), caScin hydrolysate (1000 mg/1), and 2,4-D (5 (mg/1), either alone or in combination with BA (2.5 mg/1). Three of the four embryogenic cell lines originated from one source tree and were initiated in the light, and the other was derived from a second source tree and initiated in darkness. Responsive expiants were collected between July 14 and 25, 1988, in western Washington. All four lines have been maintained for 10 or more months. Limited numbers of cotyledonary somatic embryos were obtained following transfer to a transition medium with no growth regulators and caScin hydrolysate but with L-glutamine and activated charcoal, and subsequent culture on maturation medium lacking caScin hydrolysate and supplemented with abscissic acid and L-glutamine.
2,4-dichlorophenoxy acetic acid
Basal medium of Murashige and Skoog (1962)
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