Adventitious Bud Production from Mature Picea Abies: Rejuvenation Associated with Female Strobili Formation
Developing female strobili of mature Picea abies were induced to form adventitious bud primordia when cultured on half-strength LP medium, containing 10-5 M 6-benzyl amino purine. Further development of buds required transfer to media free of growth regulators. A high frequency of strobili discs forming bud primordia was obtained only when strobili were collected at about the time of meiosis. Adventitious buds arose mainly from the outside edges of strobili discs; some also arose from the cut surface of the ovuliferous scales. The frequency of adventitious bud formation varied from tree to tree, indicating intraspecific variation in bud formation.
Unable to display preview. Download preview PDF.
- 2.Becwar, M.R., T.L. Noland and S.R. Wann. 1986. Somatic embryo development and plant regeneration from embryogenic Norway spruce callus. Tappi Research and Development Conf. Tappi Press, Atlanta, Georgia. P. 125–130.Google Scholar
- 3.Becwar, M.R., S.A. Verhange, and S.R. Wann. 1987. The frequency of plant regeneration from Norway spruce somatic embryos. Proceeding 19th Southern Forest Tree Improvement Conf. College Station, Texas. The National Technical Information Service, Springfield, Virginia. P. 92–100.Google Scholar
- 4.Bonga, J.M. 1980. Plant propagation through tissue culture, emphasizing woody species. In F. Sala, B. Parisi, R. Cella and O. Cifferi (Eds.). Plant Cell Cultures: Results and Perspectives. Elsevier/ North Holland Biomedical Press. New York. P. 253–264.Google Scholar
- 5.Bonga, J.M. 1981. Organogenesis in. vitro of tissues from mature conifers. In Vitro 17:511–518.Google Scholar
- 9.Bonga, J.M. and P. von Aderkas. 1988. Attempts to micropropagate mature Larix decidua Mill. IUFRO Workshop on somatic cell genetics of woody plants. Grosshansdorf, West Germany.Google Scholar
- 19.Karnosky, D.F. 1981. Potential for forest tree improvement via tissue culture. Bio. Sci. 31:114–120.Google Scholar
- 21.Litvay, J.D., M.A. Johnson, D. Verma, D. Einsphahr, and K. Weyrauch. 1981. Conifer suspension culture medium development using analytical data from developing seeds. Inst. Paper Chemistry, Appleton, Wis. Tech. Paper No. 115.Google Scholar
- 22.Lloyd, G.B. and B.H. McCown. 1980. Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot-tip culture. Proc. Inter. Plant Propagators Soc. 30:421–437.Google Scholar