Advertisement

Adventitious Bud Production from Mature Picea Abies: Rejuvenation Associated with Female Strobili Formation

  • Kathryn X. Wang
  • David F. Karnosky
  • Roger Timmis
Part of the NATO ASI Series book series (NSSA, volume 210)

Abstract

Developing female strobili of mature Picea abies were induced to form adventitious bud primordia when cultured on half-strength LP medium, containing 10-5 M 6-benzyl amino purine. Further development of buds required transfer to media free of growth regulators. A high frequency of strobili discs forming bud primordia was obtained only when strobili were collected at about the time of meiosis. Adventitious buds arose mainly from the outside edges of strobili discs; some also arose from the cut surface of the ovuliferous scales. The frequency of adventitious bud formation varied from tree to tree, indicating intraspecific variation in bud formation.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Ahuja, M.R. 1987. In vitro propagation of poplar and aspen. In Bonga, J.M. and D.J. Durzan (Eds.) Cell and Tissue Culture in Forestry. Vol. 3. Martinus Nijhoff Pub., Boston. P. 207–223.CrossRefGoogle Scholar
  2. 2.
    Becwar, M.R., T.L. Noland and S.R. Wann. 1986. Somatic embryo development and plant regeneration from embryogenic Norway spruce callus. Tappi Research and Development Conf. Tappi Press, Atlanta, Georgia. P. 125–130.Google Scholar
  3. 3.
    Becwar, M.R., S.A. Verhange, and S.R. Wann. 1987. The frequency of plant regeneration from Norway spruce somatic embryos. Proceeding 19th Southern Forest Tree Improvement Conf. College Station, Texas. The National Technical Information Service, Springfield, Virginia. P. 92–100.Google Scholar
  4. 4.
    Bonga, J.M. 1980. Plant propagation through tissue culture, emphasizing woody species. In F. Sala, B. Parisi, R. Cella and O. Cifferi (Eds.). Plant Cell Cultures: Results and Perspectives. Elsevier/ North Holland Biomedical Press. New York. P. 253–264.Google Scholar
  5. 5.
    Bonga, J.M. 1981. Organogenesis in. vitro of tissues from mature conifers. In Vitro 17:511–518.Google Scholar
  6. 6.
    Bonga, J.M. 1982a. Vegetative propagation in relation to juvenility, maturity and rejuvenation. In J.M. Bonga and D.J. Durzan (Eds.). Tissue Culture in Forestry. Martinus Nijhoff. Dr. W. Junk Pub. Boston. P. 387–412.CrossRefGoogle Scholar
  7. 7.
    Bonga, J.M. 1982b. Shoot formation in callus from the stalks of young female strobili of Larix decidua. Can. J. Bot. 60:1357–1359.CrossRefGoogle Scholar
  8. 8.
    Bonga, J.M. 1984. Adventitious shoot formation in cultures of immature female strobili of Larix decidua. Physiol. Plant. 62:416–421.CrossRefGoogle Scholar
  9. 9.
    Bonga, J.M. and P. von Aderkas. 1988. Attempts to micropropagate mature Larix decidua Mill. IUFRO Workshop on somatic cell genetics of woody plants. Grosshansdorf, West Germany.Google Scholar
  10. 10.
    Bornman, C.H. 1983. Possibilities and constraints in the regeneration of trees from cotyledonary needles of Picea abies in vitro. Physiol. Plant. 57:5–16.CrossRefGoogle Scholar
  11. 11.
    Boulay, M. 1987. Conifer micropropation: Applied research and commercial aspects. In J.M. Bonga and D.J. Durzan (Eds.). Cell and Tissue Culture in Forestry. Vol. 3. Martinus Nijhoff. Dr. W. Junk Pub. Boston. P. 185–206.CrossRefGoogle Scholar
  12. 12.
    Chalupa, V. 1987. European hardwoods. In J.M. Bonga and D.J. Durzan (Eds.). Cell and Tissue Culture in Forestry. Vol. 3. Martinus Nijhoff. Dr. W. Junk Pub. Boston. P. 224–246.CrossRefGoogle Scholar
  13. 13.
    Gupta, P.K. and D.J. Durzan. 1985. Shoot multiplication from mature trees of Douglas fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana). Plant Cell Rep. 4:177–179.CrossRefGoogle Scholar
  14. 14.
    Gupta, P.K., A.F. Mascarenhas, and V. Jagannathan. 1981. Tissue culture of forest trees: Propagation of mature trees of Eucalyptus citrio-dora by tissue culture. Plant Sci. Lett. 20:195–201.CrossRefGoogle Scholar
  15. 15.
    Hakman, I. and S. von Arnold. 1985. Plant let regeneration through somatic embryogenesis in Picea abies (Norway spruce). J. Plant Physiol. 121:149–158.CrossRefGoogle Scholar
  16. 16.
    Hakman, I., L.C. Fowke, S. von Arnold and T. Eriksson. 1985. The development of somatic embryos in the tissue cultures initiated from immature embryos of Picea abies (Norway spruce). Plant Sci. 88:53–59.CrossRefGoogle Scholar
  17. 17.
    Horgan, K. 1987. Pinus radiata. In J.M. Bonga and D.J. Durzan (Eds.). Cell and Tissue Culture in Forestry. Vol. 3. Martinus Nijhoff Pub. Boston. P. 128–145.CrossRefGoogle Scholar
  18. 18.
    Jain, S.M., R.J. Newton and E.J. Soltes. 1988. Enhancement of somatic embryogenesis in Norway spruce. Theor. Appl. Genet. 76:501–506.CrossRefGoogle Scholar
  19. 19.
    Karnosky, D.F. 1981. Potential for forest tree improvement via tissue culture. Bio. Sci. 31:114–120.Google Scholar
  20. 20.
    Laliberte, S. and M. LaLonde. 1988. Sustained caulogenesis in callus cultures of Larix x eurolepis initiated from short-shoot buds of a 12-year-old tree. Amer. J. Bot. 75:767–777.CrossRefGoogle Scholar
  21. 21.
    Litvay, J.D., M.A. Johnson, D. Verma, D. Einsphahr, and K. Weyrauch. 1981. Conifer suspension culture medium development using analytical data from developing seeds. Inst. Paper Chemistry, Appleton, Wis. Tech. Paper No. 115.Google Scholar
  22. 22.
    Lloyd, G.B. and B.H. McCown. 1980. Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot-tip culture. Proc. Inter. Plant Propagators Soc. 30:421–437.Google Scholar
  23. 23.
    Schenk, R.U. and A.C. Hildenbrandt. 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant-cell cultures. Can. J. Bot. 50:199–204.CrossRefGoogle Scholar
  24. 24.
    von Arnold, S. 1982. Factors influencing formation, development and rooting of adventitious shoots from embryos of Picea abies (L.) Karst. Plant Sci. Lett. 27:275–287.CrossRefGoogle Scholar
  25. 25.
    von Arnold, S. 1987. Improved efficiency of somatic erabryogenesis mature embryos of Picea abies. J. Plant Physiol. 128:233–244.CrossRefGoogle Scholar
  26. 26.
    von Arnold, S. and T. Eriksson. 1979. Bud induction on isolated needles of Norway spruce grown in vitro. Plant Sci. Lett. 15:363–372.CrossRefGoogle Scholar
  27. 27.
    von Arnold, S. and T. Eriksson. 1981. In vitro studies of adventitious shoot formation in Pinus contorta. Can. J. Bot. 59:870–874.CrossRefGoogle Scholar
  28. 28.
    von Arnold, S. and I. Hakman. 1986. Effect of sucrose on initiation of embryogenie callus from mature zygotic embryos of Picea abies (Norway spruce). J. Plant Physiol. 122:261–265.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Kathryn X. Wang
    • 1
    • 2
  • David F. Karnosky
    • 1
    • 2
  • Roger Timmis
    • 1
    • 2
  1. 1.School of Forestry and Wood ProductsMichigan Technological UniversityHoughtonUSA
  2. 2.Weyerhaeuser Company TacomaTacomaUSA

Personalised recommendations