Sequence Informative Fragmentation in 252Cf Plasma Desorption Mass Spectrometry
In the field of peptide chemistry plasma-desorption mass spectrometry (PDMS) is now fully accepted as a useful method for obtaining accurate molecular weight information on peptides up to about 25 kDa molecular weight. Furthermore it has given useful information in C-terminal sequence determination by analyzing the trunk peptide instead of the liberated amino acids . In addition, the method is very useful in peptide mapping by the method introduced by Morris et al.  applying fast atom bombardment (FAB) mass spectrometry. In these experiments PDMS has mainly been used as a technique to obtain molecular weight information. Nevertheless in some reports the presence of sequence information has been shown in smaller peptides [3–6]. During peptide mapping of recombinant IL-3 however we obtained a spectrum of a 4600 Da peptide containing considerable sequence informative fragmentation.
KeywordsHigh Performance Liquid Chromatography Ammonium Bicarbonate Fast Atom Bombardment Peptide Mapping High Energy Collision
Unable to display preview. Download preview PDF.
- 1.K. Klarskov, K. Breddam and P. Roepstorff, Anal. Biochem., 180, 28 (1989).Google Scholar
- 5.P. F. Nielsen, Thesis, Odense University, Denmark.Google Scholar
- 6.H. Lam-Thanh, C. Deprun, Y. Le Beyec, RCM vol. 4, no. 2 (1990).Google Scholar
- 10.S. A. Martin, R. S. Johnson, CE. Costello and K. Biemann in “Proceedings of the 4th Texas Symposium on Mass Spectrometry”, ed. C.J. McNeal, John Wiley and Sons, (1988).Google Scholar
- 12.I. Jardine in “Proceedings of the 4th Texas Symposium on Mass Spectrometry”, ed. C.J. McNeal, John Wiley and Sons, (1988), p. 41.Google Scholar