Competitive Peptide Adsorption to Nitrocellulose Covered Backing Material in Cf-252-Plasma Desorption Mass Spectrometry

  • G. Allmaier
  • E. Pittenauer
  • E. R. Schmid
Part of the NATO ASI Series book series (NSSB, volume 269)


Cf-252-Plasma desorption (PD) [1] and Fast atom bombardment (FAB) [2] mass spectrometry have emerged as powerful tools for the analysis of complex underivatized peptide mixtures obtained from chemical or enzymatic cleaved proteins. It is well known that suppression effects in FAB mass spectra of tryptic peptide mixtures are limiting the use of this technique [3]. This perferential desorption/ionization was mainly attributed to the different hydrophobicity of the individual compounds [4]. In fact, it turned out that the different surface excess concentrations of the individual peptides in the applied liquid matrix were responsible for the selective desorption behavior [5]. This behavior of the tryptic peptides in the mixture was correlated with their average hydrophobicities [4,5] based either on the indices of Bull and Breese [6] or of Park et al. [7]. This severe limitation can be partly reduced by HPLC separation or chemical derivatization [4].


Peptide Mixture Bovine Insulin Cyanogen Bromide Fast Atom Bombardment Mass Spectrometry Fission Event 
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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • G. Allmaier
    • 1
  • E. Pittenauer
    • 1
  • E. R. Schmid
    • 1
  1. 1.Institute for Analytical ChemistryUniversity of ViennaViennaAustria

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