On the Detectability of Low Velocity High Mass Ions in Matrix Assisted Laser Desorption TOF-MS
Within the last two years matrix assisted laser desorption mass spectrometry of high mass organic molecules has rapidly evolved. The range of useful matrices has been widely enlarged  and the demonstrated detectability now covers proteins (up to several hundred kDa), polynucleotides including large DNA fragments (see paper of P. Williams, this volume), glycoproteins and polysaccharides. The notion that this technique is restricted to UV-lasers has recently become historical after Hillenkamp et al.  have demonstrated infrared lasers (Er:YAG, CO2) to be as effective in high mass ion production as UV-lasers.
KeywordsSecondary Particle Secondary Electron Emission Transmitted Primary Surface Induce Dissociation Insulin Monomer
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