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Matrix-Assisted UV Laser Desorption with a Time Digitizer

  • W. Ens
  • Y. Mao
  • F. Mayer
  • K. G. Standing
Part of the NATO ASI Series book series (NSSB, volume 269)

Abstract

The introduction of matrix-assisted laser desorption by Karas and Hillenkamp [1] has significantly extended the mass range of proteins and peptides accessible to mass spectrometry. In this technique, a large number of molecular ions are produced for each laser shot and time-of-flight spectra are normally recorded using a transient recorder, i.e. the current is measured as a function of time. The signal-to-background ratio and the resolution have been found to improve as the power density is reduced to a “threshold” irradiance [2].

Keywords

Laser Shot Successful Event High Repetition Rate Bovine Insulin Sinapinic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    M. Karas, D. Bachmann, U. Bahr and F. Hillenkamp, Int. J. Mass Spectrom. Ion Processes 78 (1987) 53;CrossRefGoogle Scholar
  2. 1a.
    M. Karas, D. Bachmann, U. Bahr and F. Hillenkamp, Anal. Chem. 60 (1988) 2299.CrossRefGoogle Scholar
  3. 2.
    R. C. Beavis and B. T. Chait, Rapid Commun. Mass Spectrom. 3 (1989) 233, 432 and 436.CrossRefGoogle Scholar
  4. 3.
    W. Ens, Y. Mao, F. Mayer and K. G. Standing, Rapid Commun. Mass Spectrom. 5 (1991)117.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • W. Ens
    • 1
  • Y. Mao
    • 1
  • F. Mayer
    • 1
  • K. G. Standing
    • 1
  1. 1.Department of PhysicsUniversity of ManitobaWinnipegCanada

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