Matrix-Assisted UV Laser Desorption with a Time Digitizer
The introduction of matrix-assisted laser desorption by Karas and Hillenkamp  has significantly extended the mass range of proteins and peptides accessible to mass spectrometry. In this technique, a large number of molecular ions are produced for each laser shot and time-of-flight spectra are normally recorded using a transient recorder, i.e. the current is measured as a function of time. The signal-to-background ratio and the resolution have been found to improve as the power density is reduced to a “threshold” irradiance .
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